Frontiers in Microbiology (Aug 2023)

Probiotic Escherichia coli Nissle 1917-derived outer membrane vesicles modulate the intestinal microbiome and host gut-liver metabolome in obese and diabetic mice

  • Jun Shi,
  • Jun Shi,
  • DongXue Ma,
  • DongXue Ma,
  • ShanHu Gao,
  • ShanHu Gao,
  • Fei Long,
  • Fei Long,
  • Xin Wang,
  • Xin Wang,
  • XingYu Pu,
  • XingYu Pu,
  • Richard D. Cannon,
  • Ting-Li Han,
  • Ting-Li Han,
  • Ting-Li Han

DOI
https://doi.org/10.3389/fmicb.2023.1219763
Journal volume & issue
Vol. 14

Abstract

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IntroductionObesity and diabetes are common chronic metabolic disorders which can cause an imbalance of the intestinal flora and gut-liver metabolism. Several studies have shown that probiotics, including Escherichia coli Nissle 1917 (EcN), promote microbial balance and metabolic health. However, there are no studies on how EcN outer membrane vesicles (EcN-OMVs) influence the intestinal microflora and affect the metabolic disorders of obesity and diabetes.MethodsIn this study, we evaluated the effects of EcN-OMVs on high-fat diet (HFD)-induced obesity and HFD + streptozotocin (STZ)-induced diabetes.ResultsEcN-OMVs could reduce body weight, decrease blood glucose, and increase plasma insulin in obese mice. Similarly, EcN-OMVs treatment could modify the ratio of Firmicutes/Bacteroidetes in the gut, elevate intestinal short-chain fatty acid (SCFA)-producing flora, and influence the SCFA content of the intestine. Furthermore, the intestinal metabolites ornithine and fumaric acid, hepatic ω-6 unsaturated fatty acids, and SCFAs were significantly increased after administering EcN-OMVs.DiscussionOverall, this study showed that EcN-OMVs might act as post-biotic agents that could modulate gut-liver metabolism and ameliorate the pathophysiology of obesity and diabetes.

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