Generation of a luciferase-expressing human embryonic stem cell line: NERCe002-A-2

Yingying Peng; Menghan Xie; Xingxiang Duan; Liang Hu; Juan Yu; Sicong Zeng; Yang Wang; Qi Ouyang; Guangxiu Lu; Ge Lin; Yi Sun

Stem Cell Research (Apr 2018)

Generation of a luciferase-expressing human embryonic stem cell line: NERCe002-A-2

Yingying Peng,
Menghan Xie,
Xingxiang Duan,
Liang Hu,
Juan Yu,
Sicong Zeng,
Yang Wang,
Qi Ouyang,
Guangxiu Lu,
Ge Lin,
Yi Sun

Affiliations

Yingying Peng: Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China
Menghan Xie: Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China
Xingxiang Duan: Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China
Liang Hu: Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; National Engineering and Research Center of Human Stem Cells, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, China
Juan Yu: National Engineering and Research Center of Human Stem Cells, Changsha, China
Sicong Zeng: Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; National Engineering and Research Center of Human Stem Cells, Changsha, China
Yang Wang: Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China
Qi Ouyang: Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; National Engineering and Research Center of Human Stem Cells, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, China
Guangxiu Lu: National Engineering and Research Center of Human Stem Cells, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, China; Reproductive & Genetic Hospital of CITIC-Xiangya, Changsha, China
Ge Lin: Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; National Engineering and Research Center of Human Stem Cells, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, China; Reproductive & Genetic Hospital of CITIC-Xiangya, Changsha, China
Yi Sun: Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; National Engineering and Research Center of Human Stem Cells, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, China; Corresponding author at: Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China.

Journal volume & issue: Vol. 28
pp. 172 – 176

Abstract

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The human embryonic stem cell line NERCe002-A-2 was generated by transduction of NERCe002-A cells with an expression vector carrying the luciferase gene. The stem cells labelled with luciferase can be transplanted into animals and detected by the bioluminescence imaging technology. This provides optimal prospects of application to in vivo stem cell tracing. Luciferin served as a substrate to detect the activity of luciferase, and luciferase expression was measured by quantitative PCR. Characterization assays suggested that the NERCe002-A-2 cell line expresses typical markers of pluripotency and can form the 3 germ layers in vivo.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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