Effects of rosiglitazone pretreatment on blood glucose, lipid levels, urea, and microalbuminuria in rats undergoing 70% pancreatectomy

Abstract

Hasan Zafe Acar,1 Oytun Okan Senel,2 Neslihan Bukan,3 Banu Yurekli,4 A Ebru Salman,5 Fahri Yetisir61Yozgat Bozok University Hospital, General Surgery Department, Yozgat, 2Department of Surgery, Faculty of Veterinary Medicine, Ankara University, Ankara, 3Department of Medical Biochemistry, Gazi University, Ankara, 4Department of Endocrinology, Bozyaka Research and Training Hospital, Izmir, 5Department of Anesthesiology and Reanimation, 6Department of General Surgery, Ataturk Research and Training Hospital, Ankara, TurkeyBackground: Partial pancreatectomy is one of the main causes of type II diabetes, which occurs because of insulin deficiency due to a decrease in the number of β-cells. Partial pancreatectomy is the treatment of choice particularly for pancreatic cysts, benign and malign tumors of the pancreas, and pancreatic abscesses. Morbidity and mortality associated with pancreatectomy increases as a result of diabetes and its complications.Purpose: This experimental study was designed to investigate the effects of rosiglitazone pretreatment on blood glucose, lipid, urea, and microalbuminuria levels in rats undergoing 70% pancreatectomy.Material and methods: Twelve Wistar albino rats weighing 180–230 g were randomized into two groups. The study group (n = 6) received rosiglitazone (10 mg/kg/day) orally for 4 weeks before and after pancreatectomy. The control group (n = 6) received vehicle. At the end of the fourth week, all rats underwent pancreatectomy, which was performed by removing 70% of their pancreatic tissue. Blood samples were taken from the tail veins at postoperative day one, week two, and week four for analysis of blood glucose, triglyceride, cholesterol, and urea levels. Urine was collected from the bladder for microalbuminuria analysis.Results: Blood glucose levels in the study group were significantly lower than the control group at postoperative day one, week two, week four (P = 0.01, P = 0.004, and P = 0.005, respectively). Significant microalbuminuria was present at postoperative week four when compared to the control group (P < 0.05). Triglyceride, cholesterol, and urea levels were within normal limits.Conclusion: This study showed that pretreatment of rosiglitazone increased the tolerance to diabetes in rats undergoing partial pancreatectomy.Keywords: rosiglitazone, partial pancreatectomy, diabetes