Nature Communications (Jan 2019)
GORAB scaffolds COPI at the trans-Golgi for efficient enzyme recycling and correct protein glycosylation
- Tomasz M. Witkos,
- Wing Lee Chan,
- Merja Joensuu,
- Manuel Rhiel,
- Ed Pallister,
- Jane Thomas-Oates,
- A. Paul Mould,
- Alex A. Mironov,
- Christophe Biot,
- Yann Guerardel,
- Willy Morelle,
- Daniel Ungar,
- Felix T. Wieland,
- Eija Jokitalo,
- May Tassabehji,
- Uwe Kornak,
- Martin Lowe
Affiliations
- Tomasz M. Witkos
- School of Biology, Faculty of Biology, Medicine and Health, University of Manchester
- Wing Lee Chan
- Berlin-Brandenburg Centre for Regenerative Therapies (BCRT), Institut fuer Medizinische Genetik und Humangenetik, Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin and Berlin Institute of Health
- Merja Joensuu
- Cell and Molecular Biology Program, Institute of Biotechnology, University of Helsinki
- Manuel Rhiel
- Heidelberg University Biochemistry Center, Heidelberg University
- Ed Pallister
- Department of Chemistry, University of York
- Jane Thomas-Oates
- Department of Chemistry, University of York
- A. Paul Mould
- School of Biology, Faculty of Biology, Medicine and Health, University of Manchester
- Alex A. Mironov
- School of Biology, Faculty of Biology, Medicine and Health, University of Manchester
- Christophe Biot
- Univ. Lille, CNRS, UMR 8576 - UGSF - Unité de Glycobiologie Structurale et Fonctionnelle
- Yann Guerardel
- Univ. Lille, CNRS, UMR 8576 - UGSF - Unité de Glycobiologie Structurale et Fonctionnelle
- Willy Morelle
- Univ. Lille, CNRS, UMR 8576 - UGSF - Unité de Glycobiologie Structurale et Fonctionnelle
- Daniel Ungar
- Department of Biology, University of York
- Felix T. Wieland
- Heidelberg University Biochemistry Center, Heidelberg University
- Eija Jokitalo
- Cell and Molecular Biology Program, Institute of Biotechnology, University of Helsinki
- May Tassabehji
- School of Biology, Faculty of Biology, Medicine and Health, University of Manchester
- Uwe Kornak
- Berlin-Brandenburg Centre for Regenerative Therapies (BCRT), Institut fuer Medizinische Genetik und Humangenetik, Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin and Berlin Institute of Health
- Martin Lowe
- School of Biology, Faculty of Biology, Medicine and Health, University of Manchester
- DOI
- https://doi.org/10.1038/s41467-018-08044-6
- Journal volume & issue
-
Vol. 10,
no. 1
pp. 1 – 18
Abstract
COPI is recruited to the membrane by binding to Arf GTPases. Here the authors find that GORAB, a trans-Golgi protein, promotes COPI recruitment by forming membrane domains that also contain the COPI-interacting protein Scyl1, which is required for efficient glycosylation of cargo proteins.
