Spiral volumetric optoacoustic and ultrasound (SVOPUS) tomography of mice

Sandeep Kumar Kalva; Ali Özbek; Michael Reiss; Xosé Luís Deán-Ben; Daniel Razansky

Photoacoustics (Dec 2024)

Spiral volumetric optoacoustic and ultrasound (SVOPUS) tomography of mice

Sandeep Kumar Kalva,
Ali Özbek,
Michael Reiss,
Xosé Luís Deán-Ben,
Daniel Razansky

Affiliations

Sandeep Kumar Kalva: Institute of Pharmacology and Toxicology and Institute for Biomedical Engineering, Faculty of Medicine, University of Zurich, Zurich CH-8057, Switzerland; Institute for Biomedical Engineering, Department of Information Technology and Electrical Engineering, ETH Zurich, Zurich CH-8093, Switzerland; Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai 400076, India
Ali Özbek: Institute of Pharmacology and Toxicology and Institute for Biomedical Engineering, Faculty of Medicine, University of Zurich, Zurich CH-8057, Switzerland; Institute for Biomedical Engineering, Department of Information Technology and Electrical Engineering, ETH Zurich, Zurich CH-8093, Switzerland
Michael Reiss: Institute of Pharmacology and Toxicology and Institute for Biomedical Engineering, Faculty of Medicine, University of Zurich, Zurich CH-8057, Switzerland; Institute for Biomedical Engineering, Department of Information Technology and Electrical Engineering, ETH Zurich, Zurich CH-8093, Switzerland
Xosé Luís Deán-Ben: Institute of Pharmacology and Toxicology and Institute for Biomedical Engineering, Faculty of Medicine, University of Zurich, Zurich CH-8057, Switzerland; Institute for Biomedical Engineering, Department of Information Technology and Electrical Engineering, ETH Zurich, Zurich CH-8093, Switzerland
Daniel Razansky: Institute of Pharmacology and Toxicology and Institute for Biomedical Engineering, Faculty of Medicine, University of Zurich, Zurich CH-8057, Switzerland; Institute for Biomedical Engineering, Department of Information Technology and Electrical Engineering, ETH Zurich, Zurich CH-8093, Switzerland; Correspondence to: Institute of Biomedical Engineering, University of Zurich and ETH Zurich, HIT E42.1, Wolfgang-Pauli-Strasse 27, Zurich CH-8093, Switzerland.

Journal volume & issue: Vol. 40
p. 100659

Abstract

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Optoacoustic (OA) tomography is a powerful noninvasive preclinical imaging tool enabling high resolution whole-body visualization of biodistribution and dynamics of molecular agents. The technique yet lacks endogenous soft-tissue contrast, which often hampers anatomical navigation. Herein, we devise spiral volumetric optoacoustic and ultrasound (SVOPUS) tomography for concurrent OA and pulse-echo ultrasound (US) imaging of whole mice. To this end, a spherical array transducer featuring a central curvilinear segment is employed. Full rotation of the array renders transverse US and OA views, while additional translation facilitates volumetric whole-body imaging with high spatial resolution down to 150 µm and 110 µm in the OA and US modes, respectively. OA imaging revealed blood-filled, vascular organs like heart, liver, spleen, kidneys, and surrounding vasculature, whilst complementary details of bones, lungs, and skin boundaries were provided by the US. The dual-modal capability of SVOPUS for label-free imaging of tissue morphology and function is poised to facilitate pharmacokinetic studies, disease monitoring, and image-guided therapies.

Published in Photoacoustics

ISSN: 2213-5979 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Physics: Acoustics. Sound; Science: Physics: Optics. Light
Website: http://www.journals.elsevier.com/photoacoustics/

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