Nature Communications (Dec 2019)
CRISPR-Cas3 induces broad and unidirectional genome editing in human cells
- Hiroyuki Morisaka,
- Kazuto Yoshimi,
- Yuya Okuzaki,
- Peter Gee,
- Yayoi Kunihiro,
- Ekasit Sonpho,
- Huaigeng Xu,
- Noriko Sasakawa,
- Yuki Naito,
- Shinichiro Nakada,
- Takashi Yamamoto,
- Shigetoshi Sano,
- Akitsu Hotta,
- Junji Takeda,
- Tomoji Mashimo
Affiliations
- Hiroyuki Morisaka
- Department of Genome Biology, Graduate School of Medicine, Osaka University
- Kazuto Yoshimi
- Genome Editing Research and Development Center, Graduate School of Medicine, Osaka University
- Yuya Okuzaki
- Center for iPS Cell Research and Application (CiRA), Department of Clinical Application, Kyoto University
- Peter Gee
- Center for iPS Cell Research and Application (CiRA), Department of Clinical Application, Kyoto University
- Yayoi Kunihiro
- Genome Editing Research and Development Center, Graduate School of Medicine, Osaka University
- Ekasit Sonpho
- Institute of Experimental Animal Sciences, Graduate School of Medicine, Osaka University
- Huaigeng Xu
- Center for iPS Cell Research and Application (CiRA), Department of Clinical Application, Kyoto University
- Noriko Sasakawa
- Center for iPS Cell Research and Application (CiRA), Department of Clinical Application, Kyoto University
- Yuki Naito
- Database Center for Life Science
- Shinichiro Nakada
- Institute for Advanced Co-Creation Studies, Osaka University
- Takashi Yamamoto
- Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University
- Shigetoshi Sano
- Department of Dermatology, Kochi Medical School, Kochi University
- Akitsu Hotta
- Center for iPS Cell Research and Application (CiRA), Department of Clinical Application, Kyoto University
- Junji Takeda
- Department of Genome Biology, Graduate School of Medicine, Osaka University
- Tomoji Mashimo
- Genome Editing Research and Development Center, Graduate School of Medicine, Osaka University
- DOI
- https://doi.org/10.1038/s41467-019-13226-x
- Journal volume & issue
-
Vol. 10,
no. 1
pp. 1 – 13
Abstract
Class 1 CRISPR systems are not as developed for genome editing as Class 2 systems are. Here the authors show that Cas3 can be used to generate functional knockouts and knock-ins, as well as Cas3-mediated exon-skipping in DMD cells.
