Comparative estimation of metrological characteristics of different PCR types for quantitative material assessment in complex matrices on the example of soybeans’ GM line

Abstract

A method of quantitative determination of genetically modified line (GM line) of soybean Mon 89788 based on digital PCR has been developed. Comparative analysis of metrological characteristics of this technique with identical based on PCR-RT was carried out. Both the detection limit and the quantitative detection limit of the methodology were defined. Correlation coefficient (R2=0.9987) was close to the maximum value, indicating a good correlation of the analytical signal linear dependence of the GMO content in the panel samples. The digital PCR methodology allows to determine the GMO content in the range from 0.5% to 100%. Compared to real time PCR results, digital PCR results show GMO content closer to the reference value; however, this technique does not allow to determine GMO content below 0.5%.