BMC Genomics (Oct 2002)

A reference database for tumor-related genes co-expressed with interleukin-8 using genome-scale <it>in silico</it> analysis

  • Kostich Mitchell,
  • Hedrick Joseph A,
  • Gustafson Eric,
  • Bond Richard W,
  • Qiu Ping,
  • Liu Suxing,
  • Laverty Maureen,
  • Wang Lynn,
  • Benbow Lawrence,
  • Greene Jonathan R,
  • Wang Luquan

DOI
https://doi.org/10.1186/1471-2164-3-29
Journal volume & issue
Vol. 3, no. 1
p. 29

Abstract

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Abstract Background The EST database provides a rich resource for gene discovery and in silico expression analysis. We report a novel computational approach to identify co-expressed genes using EST database, and its application to IL-8. Results IL-8 is represented in 53 dbEST cDNA libraries. We calculated the frequency of occurrence of all the genes represented in these cDNA libraries, and ranked the candidates based on a Z-score. Additional analysis suggests that most IL-8 related genes are differentially expressed between non-tumor and tumor tissues. To focus on IL-8's function in tumor tissues, we further analyzed and ranked the genes in 16 IL-8 related tumor libraries. Conclusions This method generated a reference database for genes co-expressed with IL-8 and could facilitate further characterization of functional association among genes.