KLF4 recruits SWI/SNF to increase chromatin accessibility and reprogram the endothelial enhancer landscape under laminar shear stress

Jan-Renier Moonen; James Chappell; Minyi Shi; Tsutomu Shinohara; Dan Li; Maxwell R. Mumbach; Fan Zhang; Ramesh V. Nair; Joseph Nasser; Daniel H. Mai; Shalina Taylor; Lingli Wang; Ross J. Metzger; Howard Y. Chang; Jesse M. Engreitz; Michael P. Snyder; Marlene Rabinovitch

doi:10.1038/s41467-022-32566-9

Nature Communications (Aug 2022)

KLF4 recruits SWI/SNF to increase chromatin accessibility and reprogram the endothelial enhancer landscape under laminar shear stress

Jan-Renier Moonen,
James Chappell,
Minyi Shi,
Tsutomu Shinohara,
Dan Li,
Maxwell R. Mumbach,
Fan Zhang,
Ramesh V. Nair,
Joseph Nasser,
Daniel H. Mai,
Shalina Taylor,
Lingli Wang,
Ross J. Metzger,
Howard Y. Chang,
Jesse M. Engreitz,
Michael P. Snyder,
Marlene Rabinovitch

Affiliations

Jan-Renier Moonen: Vera Moulton Wall Center for Pulmonary Vascular Diseases, Stanford University School of Medicine
James Chappell: Department of Genetics, Stanford University School of Medicine
Minyi Shi: Department of Genetics, Stanford University School of Medicine
Tsutomu Shinohara: Vera Moulton Wall Center for Pulmonary Vascular Diseases, Stanford University School of Medicine
Dan Li: Vera Moulton Wall Center for Pulmonary Vascular Diseases, Stanford University School of Medicine
Maxwell R. Mumbach: Department of Genetics, Stanford University School of Medicine
Fan Zhang: Vera Moulton Wall Center for Pulmonary Vascular Diseases, Stanford University School of Medicine
Ramesh V. Nair: Stanford Center for Genomics and Personalized Medicine, Stanford University School of Medicine
Joseph Nasser: Broad Institute of MIT and Harvard
Daniel H. Mai: Department of Pediatrics, Stanford University School of Medicine
Shalina Taylor: Vera Moulton Wall Center for Pulmonary Vascular Diseases, Stanford University School of Medicine
Lingli Wang: Vera Moulton Wall Center for Pulmonary Vascular Diseases, Stanford University School of Medicine
Ross J. Metzger: Vera Moulton Wall Center for Pulmonary Vascular Diseases, Stanford University School of Medicine
Howard Y. Chang: Department of Genetics, Stanford University School of Medicine
Jesse M. Engreitz: BASE Initiative, Betty Irene Moore Children’s Heart Center, Lucile Packard Children’s Hospital, Stanford University School of Medicine
Michael P. Snyder: Stanford Cardiovascular Institute, Stanford University School of Medicine
Marlene Rabinovitch: Vera Moulton Wall Center for Pulmonary Vascular Diseases, Stanford University School of Medicine

DOI: https://doi.org/10.1038/s41467-022-32566-9
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 16

Abstract

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Here the authors studied pulmonary arterial endothelial cells (PAEC) under laminar shear stress and show that this physiologic condition markedly changes chromatin accessibility at regulatory regions, when compared to cells grown in a static state. They find that KLF4 organizes chromatin by interacting with the SWI/SNF nucleosome remodeling complex to regulate vasculo-protective gene expression.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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