Improving the activity and thermostability of PETase from Ideonella sakaiensis through modulating its post-translational glycan modification

Binyang Deng; Yu Yue; Jun Yang; Mingjun Yang; Qiong Xing; Hang Peng; Fei Wang; Ming Li; Lixin Ma; Chao Zhai

doi:10.1038/s42003-023-04413-0

Communications Biology (Jan 2023)

Improving the activity and thermostability of PETase from Ideonella sakaiensis through modulating its post-translational glycan modification

Binyang Deng,
Yu Yue,
Jun Yang,
Mingjun Yang,
Qiong Xing,
Hang Peng,
Fei Wang,
Ming Li,
Lixin Ma,
Chao Zhai

Affiliations

Binyang Deng: State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, School of Life Sciences, Hubei University
Yu Yue: State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, School of Life Sciences, Hubei University
Jun Yang: State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, School of Life Sciences, Hubei University
Mingjun Yang: State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, School of Life Sciences, Hubei University
Qiong Xing: State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, School of Life Sciences, Hubei University
Hang Peng: School of Chemistry and Chemical Engineering, Hubei University
Fei Wang: State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, School of Life Sciences, Hubei University
Ming Li: State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, School of Life Sciences, Hubei University
Lixin Ma: State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, School of Life Sciences, Hubei University
Chao Zhai: State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, School of Life Sciences, Hubei University

DOI: https://doi.org/10.1038/s42003-023-04413-0
Journal volume & issue: Vol. 6, no. 1
pp. 1 – 10

Abstract

Read online

Polyehylene terephthalate (PET) biodegradation is greatly improved when recombinant IsPETase is expressed in Pichia pastoris and the enzyme’s partial removal of N-terminal glycosylation, retaining high thermostability.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

About the journal