Effect of phytohormone balance on shoot multiplication and rooting of the species Dianthus serotinus Waldst. and Kit.

Abstract

The effect of different phytohormone concentrations on shoot multiplication and shoot rooting was researched on the species Dianthus serotinus. All nutritive media contained MS mineral solution, 3% sucrose, 0.8% agar, 100 mg·L-1 mioinositol, 0.05 mg·L-1 thiamin, 0.25 mg·L-1 nicotinic acid and 1 mg·L-1 glycine. For lateral shoot proliferation, the basic medium was supplemented with BAP (6-benzyl-aminopurine) in concentrations 0.1-2 mg·L-1 and NAA (α-naphthalene-acetic acid) in concentrations 0.1-1 mg·L-1. The best results were achieved on the medium with 0.5 mg·L-1 BAP and NAA. Rooting was most successful (73%) on the medium with 0.5 mg·L-1 NAA.

Keywords

• Dianthus serotinus
• endangered species
• propagation in vitro