Effects of breast-fed infants-derived Limosilactobacillus reuteri and Bifidobacterium breve ameliorate DSS-induced colitis in mice

Zhipeng Huang; Bingdong Liu; Lanlin Xiao; Miaomiao Liao; Liujing Huang; Xiaogan Zhao; Kai Ma; Runxin Wang; Feng Ji; Wei Li; Liping Huang; Liwei Xie

iScience (Oct 2024)

Effects of breast-fed infants-derived Limosilactobacillus reuteri and Bifidobacterium breve ameliorate DSS-induced colitis in mice

Zhipeng Huang,
Bingdong Liu,
Lanlin Xiao,
Miaomiao Liao,
Liujing Huang,
Xiaogan Zhao,
Kai Ma,
Runxin Wang,
Feng Ji,
Wei Li,
Liping Huang,
Liwei Xie

Affiliations

Zhipeng Huang: Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China; State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China
Bingdong Liu: State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China; Department of Endocrinology and Metabolism, Zhujiang Hospital, Southern Medical University, Guangzhou, China
Lanlin Xiao: State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China
Miaomiao Liao: State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China
Liujing Huang: Medical Affairs Department, Guangzhou Betrue Technology Co., Ltd. Guangzhou 510700, China
Xiaogan Zhao: Nanjing Agricultural University College of Food Science and Technology, Nanjing, Jiangsu, China
Kai Ma: Jiangsu New-bio Biotechnology Co., Ltd., Jiangyin, China
Runxin Wang: Jiangsu New-bio Biotechnology Co., Ltd., Jiangyin, China
Feng Ji: Jiangsu New-bio Biotechnology Co., Ltd., Jiangyin, China
Wei Li: Nanjing Agricultural University College of Food Science and Technology, Nanjing, Jiangsu, China; Corresponding author
Liping Huang: Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China; Corresponding author
Liwei Xie: State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China; College of Life and Health Sciences, Guangdong Industry Polytechnic University, Guangzhou, Guangdong 510300, China; Department of Endocrinology and Metabolism, Zhujiang Hospital, Southern Medical University, Guangzhou, China; Department of Internal Medicine, Shunde Women and Children’s Hospital (Maternity and Child Healthcare Hospital of Shunde Foshan), Guangdong Medical University, Foshan, Guangdong, China; Corresponding author

Journal volume & issue: Vol. 27, no. 10
p. 110902

Abstract

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Summary: Studies have shown that breastfeeding can reduce the risk and severity of inflammatory bowel disease (IBD) in children and adults. Probiotics in breast milk have also been isolated and their effects on IBD have been studied. However, based on current evidence, the exact efficacy and mechanisms of probiotics in the treatment of IBD cannot be determined. In this study, Bifidobacterium breve FPHC4024 (BB FPHC4024) and Limosilactobacillus reuteri FPHC2951 (LR FPHC2951) were isolated from feces of exclusively breastfed healthy infants and administered by gavage to dextran sulfate sodium (DSS)-induced IBD mice. The results showed that LR FPHC2951 improved the symptoms of DSS-induced IBD, increased the expression of interleukin (IL)-10 mRNA and upregulated the abundance of Verrucomicrobiaceae Akkermansia. Combined with Kyoto Encyclopedia of Genes and Genomes (KEGG)-based Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) function prediction results, we hypothesized that LR FPHC2951 improved DSS-induced colitis symptoms in mice by increasing of IL-10 mRNA, altering the structure of intestinal flora, and reducing proinflammatory pathways and enhancing pathways associated with anti-inflammatory and intestinal protection.

Published in iScience

ISSN: 2589-0042 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science
Website: http://www.cell.com/iscience/home

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