Hepatoprotective effect of Cocculus hirsutus on bile duct ligation-induced liver fibrosis in Albino Wistar rats

Abstract

In this animal model (Wistar rats of either sex) common bile duct was ligated for 28 days. Rats were treated for 28 days with methanol extract of Cocculus hirsutus. On day 29, blood and liver were collected for biochemical estimation and histopathological studies. Bile duct ligation produced liver fibrosis with generation reactive oxygen species and induction of oxidative stress hence the different concentrations of methanolic extract of C. hirsutus were evaluated for in vivo glutathione reductase activity. On bile duct ligation the liver fibrosis was induced with significant rise in serum marker enzymes levels. The hydroxyproline accumulation caused by hydrophilic bile acids accompanied by elevated hepatic lipid peroxidation, and glutathione levels. Treatment with C. hirsutus decreased the elevated levels of serum marker enzymes showing hepatoprotection, which was further confirmed by histopathological results.

Keywords

• Bile duct ligation
• Cocculus hirsutus
• Glutathione
• Hepatoprotective activity
• Oxidative stress