CryoEM structure of the type IVa pilus secretin required for natural competence in Vibrio cholerae

Sara J. Weaver; Davi R. Ortega; Matthew H. Sazinsky; Triana N. Dalia; Ankur B. Dalia; Grant J. Jensen

doi:10.1038/s41467-020-18866-y

Nature Communications (Oct 2020)

CryoEM structure of the type IVa pilus secretin required for natural competence in Vibrio cholerae

Sara J. Weaver,
Davi R. Ortega,
Matthew H. Sazinsky,
Triana N. Dalia,
Ankur B. Dalia,
Grant J. Jensen

Affiliations

Sara J. Weaver: Division of Chemistry and Chemical Engineering, California Institute of Technology
Davi R. Ortega: Division of Biology and Biological Engineering and Howard Hughes Medical Institute, California Institute of Technology
Matthew H. Sazinsky: Department of Chemistry, Pomona College
Triana N. Dalia: Department of Biology, Indiana University
Ankur B. Dalia: Department of Biology, Indiana University
Grant J. Jensen: Division of Biology and Biological Engineering and Howard Hughes Medical Institute, California Institute of Technology

DOI: https://doi.org/10.1038/s41467-020-18866-y
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 13

Abstract

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In Vibrio cholerae, a type IVa pilus (T4aP) binds to exogenous DNA, and threads this DNA through the outer membrane secretin, PilQ. Here authors present the cryoEM structure of PilQ from native V. cholerae cells and design a series of mutants to reversibly regulate VcPilQ gate dynamics.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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