A Cas9 with PAM recognition for adenine dinucleotides

Pranam Chatterjee; Jooyoung Lee; Lisa Nip; Sabrina R. T. Koseki; Emma Tysinger; Erik J. Sontheimer; Joseph M. Jacobson; Noah Jakimo

doi:10.1038/s41467-020-16117-8

Nature Communications (May 2020)

A Cas9 with PAM recognition for adenine dinucleotides

Pranam Chatterjee,
Jooyoung Lee,
Lisa Nip,
Sabrina R. T. Koseki,
Emma Tysinger,
Erik J. Sontheimer,
Joseph M. Jacobson,
Noah Jakimo

Affiliations

Pranam Chatterjee: Center for Bits and Atoms
Jooyoung Lee: RNA Therapeutics Institute, University of Massachusetts Medical School
Lisa Nip: Center for Bits and Atoms
Sabrina R. T. Koseki: Center for Bits and Atoms
Emma Tysinger: Center for Bits and Atoms
Erik J. Sontheimer: RNA Therapeutics Institute, University of Massachusetts Medical School
Joseph M. Jacobson: Center for Bits and Atoms
Noah Jakimo: Center for Bits and Atoms

DOI: https://doi.org/10.1038/s41467-020-16117-8
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 6

Abstract

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Protospacer adjacent motif (PAM) requirements limit the target range of CRISPR endonucleases. Here, the authors graft the 5$$^{\prime}$$ ′ -NAAN-3$$^{\prime}$$ ′ PAM-interacting domain of SmacCas9 onto SpyCas9 to create adenine dinucleotide targeting chimeras.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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