Research Regarding the New Biodiversity Indicators in Genetic Diversity of the Region -Country Hateg

Abstract

The present research started in June 2009 by identification of the species and breeds in the livestock of the 11 villages and 1 town existing in the Hatseg Land area. We use the modern genotyping tool for the study of zootechnical biodiversity- molecular biology tests- based on identification, amplification and characterization of nucleic acid, revolutionized the conservation of indigene animal genetic resources, gene assisted selection, pathology diagnostic and food traceability. The original Tipy Fix methods – internatinal patented by Prof.Brem - that were used in Romania (using for the first time in Romania by the researchers of CSCBA) to reveal DNA polymorphism are described as their applicability in species identification and meat traceability. Vulnerability of farm animal breeds is caused by the lack of interest apart breeders for one breed. In Hateg land area the main mean of reducing biodiversity in farm animals is the crossbreeding. It was analysis the prion protein for scrapie resistance genotyping as codon- amino acid at codon 136, 154, 171 from 5 known haplotypes resulting PrP Genotype .In results of analysis in Hateg country 41 the probes present the arginine (R) at codon 171 of the prion protein who confers resistance to the structural change of prion scrapie.We presented biodiversity indicator for domestic animal in Hateg country.

Keywords

• genotipying
• prion protein
• tipy fix methods
• zootechnical biodiversity