Imaging multicellular specimens with real-time optimized tiling light-sheet selective plane illumination microscopy

Qinyi Fu; Benjamin L. Martin; David Q. Matus; Liang Gao

doi:10.1038/ncomms11088

Nature Communications (Mar 2016)

Imaging multicellular specimens with real-time optimized tiling light-sheet selective plane illumination microscopy

Qinyi Fu,
Benjamin L. Martin,
David Q. Matus,
Liang Gao

Affiliations

Qinyi Fu: Department of Chemistry, Stony Brook University
Benjamin L. Martin: Department of Biochemistry & Cell Biology, Stony Brook University
David Q. Matus: Department of Biochemistry & Cell Biology, Stony Brook University
Liang Gao: Department of Chemistry, Stony Brook University

DOI: https://doi.org/10.1038/ncomms11088
Journal volume & issue: Vol. 7, no. 1
pp. 1 – 10

Abstract

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Selective plane illumination microscopy (SPIM) is capable of high-resolution, high-speed 3D imaging of single cells, but application to multicellular samples is challenging. Here the authors develop tiling light sheet SPIM to image large multicellular specimens in 3D with subcellular resolution.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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