Vіsnik Naukovih Doslіdžen' (Nov 2017)
PECULIARITIES OF ENERGY SUPPLY IN THE WALL OF THE SMALL INTESTINE AT CHRONIC ENTEROCOLITIS COMBINED WITH STREPTOZOTOCIN-INDUCED DIABETES IN RATS
Abstract
Despite the diversity of the studied pathologies pathogenesis, the development of chronic inflammation due to a violation of intestinal metabolism, according to some authors, is crucial in chronic enterocolitis and diabetes mellitus. The aim of the study – to find out the state of energy supply in the wall of the small intestine in chronic enterocolitis combined with streptozotocin diabetes. Materials and Metods. The study was conducted on 48 white non-linear male rats in accordance with the principles of the European Convention for the Protection of Laboratories. Diabetes mellitus (DM ) was modeled by a single intraperitoneal administration of streptozotocin to animals aged 2 months, (Sigma Aldrich, USA , at a dose of 60 mg / kg of body weight). Chronic enterocolitis (CEC) was induced by a free access of animals to 1.0 % solution of carrageenan in drinking water for 1 month. The activity of succinate dehydrogenase (SD ), cytochrome oxidase (CO) and transaminases was performed according to standard methods. Results and Discussion. A significant increasing in the activity of aminotransferases in the small intestine homogenate at DM was established, whereas in the case of CEC only AL aT activity was statistically significantly increased. It should be noted that at CEC combined with STD , the highest rates of aminotransferase activity are established. It was established that under the condition of simulated CEC on the background of STD in the mitochondria of the muscular layer of the wall of the small intestine there is a decrease in the activity of SD at 44.78 % and CO – 33.18 %, against the data of control (p <0.01). At the same time, the animals with CEC on the background of DM showed the maximum energy deficit, which was statistically significantly higher than the results of the studied indicators for CEC (SD at 14.50 % and CO – 8.40 %) and DM (SD at 29.59 % and CO – on 19.21%). The received changes in the activity of SD and CO indicate the inhibition of the mitochondria function in enterocytes in all studied pathologies, which may be one of the causes of increased activity of aminotransferases due to cell damage. Conclusions. In case of chronic enterocolitis combined with streptozotocin diabetes, the intensification of oxidative destruction processes is accompanied by changes in the enzymatic activity of energy supply indices (reduction of the activity of succinate dehydrogenase by 44.8 % and cytochrome oxidase by 33.2 %), which leads to destructive damage of the small intestine cells (increased activity of AlAT by 32.02 % and AsAT by 20.47 %), p<0.01.
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