Extracellular Vesicles Secreted by Human Adipose-derived Stem Cells (hASCs) Improve Survival Rate of Rats with Acute Liver Failure by Releasing lncRNA H19

Yinpeng Jin; Junyi Wang; Hongchao Li; Shane Gao; Rongfeng Shi; Danjing Yang; Xianli Wang; Xi Wang; Liang Zhu; Xiaojin Wang; Chengwei Chen; Ke Ning; Qingchun Fu; Jun Xu; Zhengliang Gao

EBioMedicine (Aug 2018)

Extracellular Vesicles Secreted by Human Adipose-derived Stem Cells (hASCs) Improve Survival Rate of Rats with Acute Liver Failure by Releasing lncRNA H19

Yinpeng Jin,
Junyi Wang,
Hongchao Li,
Shane Gao,
Rongfeng Shi,
Danjing Yang,
Xianli Wang,
Xi Wang,
Liang Zhu,
Xiaojin Wang,
Chengwei Chen,
Ke Ning,
Qingchun Fu,
Jun Xu,
Zhengliang Gao

Affiliations

Yinpeng Jin: Shanghai Public Health Clinical Center, Fudan University, Jinshan, Shanghai 201508, PR China; Corresponding authors.
Junyi Wang: Shanghai Liver Diseases Research Center, The 85th Hospital of PLA, Shanghai 200235, PR China
Hongchao Li: Shanghai Liver Diseases Research Center, The 85th Hospital of PLA, Shanghai 200235, PR China
Shane Gao: East Hospital, Tongji University School of Medicine, Shanghai 200120, PR China
Rongfeng Shi: Department of Interventional & Vascular Surgery, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, 200072, PR China
Danjing Yang: East Hospital, Tongji University School of Medicine, Shanghai 200120, PR China
Xianli Wang: Institute of Neuroscience and State Key Laboratory of Neuroscience, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, PR China
Xi Wang: Shanghai Public Health Clinical Center, Fudan University, Jinshan, Shanghai 201508, PR China
Liang Zhu: East Hospital, Tongji University School of Medicine, Shanghai 200120, PR China
Xiaojin Wang: Shanghai Liver Diseases Research Center, The 85th Hospital of PLA, Shanghai 200235, PR China
Chengwei Chen: Shanghai Liver Diseases Research Center, The 85th Hospital of PLA, Shanghai 200235, PR China
Ke Ning: Department of Neuroscience, Sheffield Institute for Translational Neuroscience (SITraN), University of Sheffield, Sheffield, UK
Qingchun Fu: Shanghai Public Health Clinical Center, Fudan University, Jinshan, Shanghai 201508, PR China; Corresponding authors.
Jun Xu: East Hospital, Tongji University School of Medicine, Shanghai 200120, PR China; Corresponding authors.
Zhengliang Gao: East Hospital, Tongji University School of Medicine, Shanghai 200120, PR China; Corresponding authors.

Journal volume & issue: Vol. 34
pp. 231 – 242

Abstract

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It has previously been reported that human adipose-derived stem cells (hASCs) can promote the regeneration of damaged tissues in rats with liver failure through a ‘paracrine effect’. Here we demonstrate a therapeutic effect of hASCs derived Extracellular Vesicles (EVs) on rat models with acute liver failure, as shown by the improvement of the survival rate by >70% compared to controls. Gene sequencing of rat liver revealed an increase in human long-chain non-coding RNA (lncRNA) H19 after hASC-derived EVs transplantation. When the H19 coding sequence was silenced in hASCs and EVs were then collected for treatment of rats with liver failure, we saw a decrease in the survival rate to 40%, compared to treatment with EVs generated from non-silenced hASCs. These data indicate that lncRNA H19 may be a potential therapeutic target for the treatment of liver failure. Keywords: Extracellular vesicles, Adipose-derived stem cells, Regeneration, Liver failure, LncRNA

Published in EBioMedicine

ISSN: 2352-3964 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Medicine: Medicine (General)
Website: http://www.journals.elsevier.com/ebiomedicine/

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