Uptake and metabolism of 2,4-dichlorophenoxyacetic acid during induction of Norway spruce (Picea abies (L.) Karst.) somatic embryogenesis

Abstract

Uptake and metabolism of 2,4-dichlorophenoxyacetic acid (2,4-D) into zygotic embryos of Norway spruce (Picea abies (L.) Karst.) were studied on medium used for induction of somatic embryogenesis. The main task of this work was to study effect of longitudinal bisection of embryos, which was found as increasing the subsequent induction of embryogenic cultures. The maximal uptake of 2,4-D per one embryo was detected after 16 hours on medium in bisected embryos. The bisection increased 2,4-D uptake per embryo in first 16th hours, but then increased its release back to the medium. The metabolism of 2,4-D in bisected embryos was lower than in intact ones during first two days of culture.

Keywords

• 2,4-dichlorophenoxyacetic acid
• Norway spruce
• somatic embryogenesis