Small molecule regulated sgRNAs enable control of genome editing in E. coli by Cas9

Roman S. Iwasaki; Bagdeser A. Ozdilek; Andrew D. Garst; Alaksh Choudhury; Robert T. Batey

doi:10.1038/s41467-020-15226-8

Nature Communications (Mar 2020)

Small molecule regulated sgRNAs enable control of genome editing in E. coli by Cas9

Roman S. Iwasaki,
Bagdeser A. Ozdilek,
Andrew D. Garst,
Alaksh Choudhury,
Robert T. Batey

Affiliations

Roman S. Iwasaki: Department of Biochemistry, University of Colorado at Boulder
Bagdeser A. Ozdilek: Department of Biochemistry, University of Colorado at Boulder
Andrew D. Garst: Department of Chemical and Biological Engineering, University of Colorado at Boulder
Alaksh Choudhury: Department of Chemical and Biological Engineering, University of Colorado at Boulder
Robert T. Batey: Department of Biochemistry, University of Colorado at Boulder

DOI: https://doi.org/10.1038/s41467-020-15226-8
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 9

Abstract

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Bacteria lack the same suite of CRISPR tools that have been developed for mammalian cells. Here, the authors link aptamers to sgRNAs to allow small molecule control of gene editing in E. coli.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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