Revista da Sociedade Brasileira de Medicina Tropical (Dec 2015)

Molecular detection of Trypanosoma sp. and Blastocrithidia sp. (Trypanosomatidae) in phlebotomine sand flies (Psychodidae) in the Federal District of Brazil

  • Tauana de Sousa Ferreira,
  • Thaís Tâmara Castro Minuzzi-Souza,
  • Andrey José de Andrade,
  • Thais Oliveira Coelho,
  • Douglas de Almeida Rocha,
  • Marcos Takashi Obara,
  • Mariana Hecht,
  • Nadjar Nitz,
  • Rodrigo Gurgel-Gonçalves

DOI
https://doi.org/10.1590/0037-8682-0076-2015
Journal volume & issue
Vol. 48, no. 6
pp. 776 – 779

Abstract

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Abstract: INTRODUCTION : This study describes the occurrence of trypanosomatids in phlebotomines in Brasília, Brazil. METHODS : Two hundred and ten females of 13 sand fly species were analyzed by polymerase chain reaction (PCR) using different molecular markers (D7 24Sα rRNA, kDNA, and ITS1) and sequencing. RESULTS : PCR revealed trypanosomatid-positive samples from Nyssomyia whitmani and Evandromyia evandroi, which were negative by kDNA and ITS1 Leishmania-specific PCRs. DNA sequence analysis of D7 24Sα rRNA amplicons indicated the occurrence of Blastocrithidia sp. and Trypanosoma sp. in Nyssomyia whitmani and Evandromyia evandroi, respectively. CONCLUSIONS : Two trypanosomatid species other than Leishmania sp. were found to circulate in sand flies in Central Brazil.

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