Phosphoproteomics identifies a bimodal EPHA2 receptor switch that promotes embryonic stem cell differentiation

Rosalia Fernandez-Alonso; Francisco Bustos; Manon Budzyk; Pankaj Kumar; Andreas O. Helbig; Jens Hukelmann; Angus I. Lamond; Fredrik Lanner; Houjiang Zhou; Evangelia Petsalaki; Greg M. Findlay

doi:10.1038/s41467-020-15173-4

Nature Communications (Mar 2020)

Phosphoproteomics identifies a bimodal EPHA2 receptor switch that promotes embryonic stem cell differentiation

Rosalia Fernandez-Alonso,
Francisco Bustos,
Manon Budzyk,
Pankaj Kumar,
Andreas O. Helbig,
Jens Hukelmann,
Angus I. Lamond,
Fredrik Lanner,
Houjiang Zhou,
Evangelia Petsalaki,
Greg M. Findlay

Affiliations

Rosalia Fernandez-Alonso: MRC Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences, University of Dundee
Francisco Bustos: MRC Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences, University of Dundee
Manon Budzyk: MRC Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences, University of Dundee
Pankaj Kumar: Department of Clinical Science, Intervention and Technology, Ming Wai Lau Center for Reparative Medicine, Division of Obstetrics and Gynecology, Karolinska Institutet
Andreas O. Helbig: Institute for Experimental Medicine, Christian Albrechts University
Jens Hukelmann: Centre for Gene Regulation and Expression, School of Life Sciences, University of Dundee
Angus I. Lamond: Centre for Gene Regulation and Expression, School of Life Sciences, University of Dundee
Fredrik Lanner: Department of Clinical Science, Intervention and Technology, Ming Wai Lau Center for Reparative Medicine, Division of Obstetrics and Gynecology, Karolinska Institutet
Houjiang Zhou: MRC Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences, University of Dundee
Evangelia Petsalaki: European Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Genome Campus
Greg M. Findlay: MRC Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences, University of Dundee

DOI: https://doi.org/10.1038/s41467-020-15173-4
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 13

Abstract

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Fgf4 is a critical signal driving embryonic stem cell (ESC) exit from pluripotency and differentiation. Here the authors identify EPHA2 as a target of FGF4 signalling in ESCs, and show that EPHA2-EFNA1 signalling promotes pluripotent gene expression and suppresses commitment through repression of ERK1/2 activation.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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