Nature Communications (May 2021)

Microfluidic platform accelerates tissue processing into single cells for molecular analysis and primary culture models

  • Jeremy A. Lombardo,
  • Marzieh Aliaghaei,
  • Quy H. Nguyen,
  • Kai Kessenbrock,
  • Jered B. Haun

DOI
https://doi.org/10.1038/s41467-021-23238-1
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 15

Abstract

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Existing methods for tissue dissociation are inefficient and lead to variable outcomes and biases. Here, the authors present a microfluidic platform that combines digestion, disaggregation and filtration of tissue to allow single cell analysis and RNA sequencing.