Inhibitory Effect of Trimidox on Lipopolysaccharide-Induced Nitric Oxide Production in RAW 264.7 Macrophages

Syu-ichi Kanno; Mai Kakuta; Yasue Kitajima; Yuu Osanai; Kaori Kurauchi; Takaharu Ohtake; Mayuko Ujibe; Kouji Uwai; Mituhiro Takeshita; Masaaki Ishikawa

Journal of Pharmacological Sciences (Jan 2007)

Inhibitory Effect of Trimidox on Lipopolysaccharide-Induced Nitric Oxide Production in RAW 264.7 Macrophages

Syu-ichi Kanno,
Mai Kakuta,
Yasue Kitajima,
Yuu Osanai,
Kaori Kurauchi,
Takaharu Ohtake,
Mayuko Ujibe,
Kouji Uwai,
Mituhiro Takeshita,
Masaaki Ishikawa

Affiliations

Syu-ichi Kanno: Department of Clinical Pharmacotheraputics, 4-4-1 Komatsushima, Aoba-ku, Sendai 981-8558, Japan; Corresponding author. [email protected]
Mai Kakuta: Department of Clinical Pharmacotheraputics, 4-4-1 Komatsushima, Aoba-ku, Sendai 981-8558, Japan
Yasue Kitajima: Department of Clinical Pharmacotheraputics, 4-4-1 Komatsushima, Aoba-ku, Sendai 981-8558, Japan
Yuu Osanai: Department of Clinical Pharmacotheraputics, 4-4-1 Komatsushima, Aoba-ku, Sendai 981-8558, Japan
Kaori Kurauchi: Department of Clinical Pharmacotheraputics, 4-4-1 Komatsushima, Aoba-ku, Sendai 981-8558, Japan
Takaharu Ohtake: Department of Clinical Pharmacotheraputics, 4-4-1 Komatsushima, Aoba-ku, Sendai 981-8558, Japan
Mayuko Ujibe: Department of Clinical Pharmacotheraputics, 4-4-1 Komatsushima, Aoba-ku, Sendai 981-8558, Japan
Kouji Uwai: Department of Pharmaceutics, Tohoku Pharmaceutical University, 4-4-1 Komatsushima, Aoba-ku, Sendai 981-8558, Japan
Mituhiro Takeshita: Department of Pharmaceutics, Tohoku Pharmaceutical University, 4-4-1 Komatsushima, Aoba-ku, Sendai 981-8558, Japan
Masaaki Ishikawa: Department of Clinical Pharmacotheraputics, 4-4-1 Komatsushima, Aoba-ku, Sendai 981-8558, Japan

Journal volume & issue: Vol. 104, no. 3
pp. 278 – 281

Abstract

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We examined the effect of trimidox (3,4,5-trihydroxybenzamidoxime) on the production of nitric oxide (NO) by lipopolysaccharide (LPS) in mouse RAW 264.7 macrophages. Trimidox (50 – 300 µM) concentration-dependently inhibited NO production by LPS (0.01, 0.1, or 1 µg/ml) after incubation for 24 h. LPS-induced expression of inducible NO synthase (iNOS) and degradation of IκBα were prevented by trimidox. The protective effect against NO production by LPS was not only observed in prior incubation but also later incubation with trimidox until iNOS was activated by LPS. These results suggest that trimidox has a predominantly protective effect against LPS-induced production of NO via iNOS expression. Keywords:: trimidox, nitric oxide, macrophage

Published in Journal of Pharmacological Sciences

ISSN: 1347-8613 (Print)
Publisher: Elsevier
Country of publisher: Japan
LCC subjects: Medicine: Therapeutics. Pharmacology
Website: http://www.journals.elsevier.com/journal-of-pharmacological-sciences

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