New MIP-based Electrochemical Device for On-site Detection of Glucose Oxidase

Abstract

The measurement of protein concentration is a fundamental assay in diverse areas, such as medicine, environmental control and also in food industry, for monitoring enzyme content in consumer goods. In this work, the design and synthesis of artificial receptors, based on molecular imprinting (MI) technology, is described for the development of a simple and efficient electrochemical MIP-based biosensor for detection of glucose oxidase (GOD). Disposable and cost-effective chips, consisting of carbon screen-printed electrodes (C-SPEs), were used to develop the portable electrochemical devices for on-site monitoring of GOD in industrial context. MIP receptor films were created by electropolymerization of caffeic acid (CAF), in the presence of GOD as template biomolecule, at the carbon electrode surface, followed by enzyme wash-out from the polymeric matrix in order to create the specific binding sites. After that, specific binding properties between the MIP film surface and the template protein were observed. The developed MIP biosensor exhibited a very wide working concentration range, between 10-9 to 10-3 g L- 1, with a limit of detection (LOD) of 1.0´10-10 g L-1. Moreover, selectivity studies were performed against other proteins (amylase and myoglobin) revealing that the MIP surface has high specificity for GOD.

Keywords

• glucose oxidase (god)
• caffeic acid (caf)
• electrochemical biosensor
• molecularly imprinted polymer (mip)
• carbon
• screen-printed electrode (spe)