Tannins, novel inhibitors of the volume regulation and the volume-sensitive anion channel

Tsiferova N.A.; Khamidova O. J.; Amonov A. U.; Rakhimova M. B.; Rustamova S. I.; Kurbannazaova R. Sh.; Merzlyak P. G.; Abdulladjanova N. G.; Sabirov R. Z.

doi:10.2478/afpuc-2019-0016

European Pharmaceutical Journal (Nov 2019)

Tannins, novel inhibitors of the volume regulation and the volume-sensitive anion channel

Tsiferova N.A.,
Khamidova O. J.,
Amonov A. U.,
Rakhimova M. B.,
Rustamova S. I.,
Kurbannazaova R. Sh.,
Merzlyak P. G.,
Abdulladjanova N. G.,
Sabirov R. Z.

Affiliations

Tsiferova N.A.: Institute of Biophysics and Biochemistry, National University of Uzbekistan, Tashkent, Uzbekistan
Khamidova O. J.: Institute of Biophysics and Biochemistry, National University of Uzbekistan, Tashkent, Uzbekistan
Amonov A. U.: Institute of Biophysics and Biochemistry, National University of Uzbekistan, Tashkent, Uzbekistan
Rakhimova M. B.: Institute of Biophysics and Biochemistry, National University of Uzbekistan, Tashkent, Uzbekistan
Rustamova S. I.: Institute of Biophysics and Biochemistry, National University of Uzbekistan, Tashkent, Uzbekistan
Kurbannazaova R. Sh.: Institute of Biophysics and Biochemistry, National University of Uzbekistan, Tashkent, Uzbekistan
Merzlyak P. G.: Institute of Biophysics and Biochemistry, National University of Uzbekistan, Tashkent, Uzbekistan
Abdulladjanova N. G.: Institute of Bioorganic Chemistry, Academy of Sciences of Uzbekistan, Tashkent, Uzbekistan
Sabirov R. Z.: Institute of Biophysics and Biochemistry, National University of Uzbekistan, Tashkent, Uzbekistan

DOI: https://doi.org/10.2478/afpuc-2019-0016
Journal volume & issue: Vol. 66, no. 2
pp. 37 – 44

Abstract

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The volume-sensitive outwardly rectifying anion channel (VSOR) is a key component of volume regulation system critical for cell survival in non-isosmotic conditions. The aim of the present study was to test the effects of four tannin extracts with defined compositions on cell volume regulation and VSOR. Preparation I (98% of hydrolysable tannins isolated from leaves of sumac Rhus typhina L.) and Preparation II (100% of hydrolysable tannins isolated from leaves of broadleaf plantain Plantago major L) completely and irreversibly abolished swelling-activated VSOR currents in HCT116 cells. Both preparations profoundly suppressed the volume regulation in thymocytes with half-maximal effects of 40.9 μg/ml and 12.3 μg/ml, respectively. The inhibition was more efficient at lower concentrations but reverted at higher doses due to possible non-specific membrane-permeabilizing activity. Preparations III and IV (54,7% and 54.3% of hydrolysable tannins isolated, respectively, from roots and aboveground parts of Fergana spurge Euphorbia ferganensis B.Fedtch) inhibited VSOR activity in a partially reversible manner and suppressed the volume regulation with substantially higher half-maximal doses of 270 and 278 μg/ml, respectively, with no secondary reversion at higher doses. Hydrolysable tannins represent a novel class of VSOR channel inhibitors with the capacity to suppress the cell volume regulation machinery.

Published in European Pharmaceutical Journal

ISSN: 2453-6725 (Online)
Publisher: Sciendo
Country of publisher: Poland
LCC subjects: Medicine: Therapeutics. Pharmacology
Website: https://sciendo.com/journal/AFPUC

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