Biosensing of DNA oxidative damage: a model of using glucose meter for non-glucose biomarker detection

Abstract

Xuena Zhu,1 Mehenur Sarwar,1 Qiaoli Yue,2 Chunying Chen,3 Chen-Zhong Li1,4 1Nanobioengineering/Bioelectronics Laboratory, Department of Biomedical Engineering, Florida International University, Miami, FL, USA; 2Department of Chemistry, College of Chemistry and Chemical Engineering, Liao Chen University, Shandong, 3CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, Beijing, 4Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi’an, People’s Republic of China Abstract: Non-glucose biomarker-DNA oxidative damage biomarker 8-hydroxy-2'-deoxyguanosine (8-OHdG) has been successfully detected using a smartphone-enabled glucose meter. Through a series of immune reactions and enzymatic reactions on a solid lateral flow platform, 8-OHdG concentration has been converted to a relative amount of glucose, and therefore can be detected by conventional glucose meter directly. The device was able to detect 8-OHdG concentrations in phosphate buffer saline as low as 1.73 ng mL-1 with a dynamic range of 1–200 ng mL-1. Considering the inherent advantages of the personal glucose meter, the demonstration of this device, therefore, should provide new opportunities for the monitoring of a wide range of biomarkers and various target analytes in connection with different molecular recognition events. Keywords: 8-OHdG, immunostrip, point-of-care, POCTs, biosensor, DNA oxidative damage 

Keywords

• 8-OHdG
• Immunostrip
• Point-of-care
• POCTs
• Biosensor
• DNA oxidative damage