Chemical combinations potentiate human pluripotent stem cell-derived 3D pancreatic progenitor clusters toward functional β cells

Haisong Liu; Ronghui Li; Hsin-Kai Liao; Zheying Min; Chao Wang; Yang Yu; Lei Shi; Jiameng Dan; Alberto Hayek; Llanos Martinez Martinez; Estrella Nuñez Delicado; Juan Carlos Izpisua Belmonte

doi:10.1038/s41467-021-23525-x

Nature Communications (Jun 2021)

Chemical combinations potentiate human pluripotent stem cell-derived 3D pancreatic progenitor clusters toward functional β cells

Haisong Liu,
Ronghui Li,
Hsin-Kai Liao,
Zheying Min,
Chao Wang,
Yang Yu,
Lei Shi,
Jiameng Dan,
Alberto Hayek,
Llanos Martinez Martinez,
Estrella Nuñez Delicado,
Juan Carlos Izpisua Belmonte

Affiliations

Haisong Liu: Gene Expression Laboratory, The Salk Institute for Biological Studies
Ronghui Li: Gene Expression Laboratory, The Salk Institute for Biological Studies
Hsin-Kai Liao: Gene Expression Laboratory, The Salk Institute for Biological Studies
Zheying Min: Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology and Key Laboratory of Assisted Reproduction, Ministry of Education, Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital
Chao Wang: Gene Expression Laboratory, The Salk Institute for Biological Studies
Yang Yu: Gene Expression Laboratory, The Salk Institute for Biological Studies
Lei Shi: Gene Expression Laboratory, The Salk Institute for Biological Studies
Jiameng Dan: Gene Expression Laboratory, The Salk Institute for Biological Studies
Alberto Hayek: Department of Pediatrics, UCSD-Medical School
Llanos Martinez Martinez: Universidad Católica San Antonio de Murcia
Estrella Nuñez Delicado: Universidad Católica San Antonio de Murcia
Juan Carlos Izpisua Belmonte: Gene Expression Laboratory, The Salk Institute for Biological Studies

DOI: https://doi.org/10.1038/s41467-021-23525-x
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 10

Abstract

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Human pluripotent stem cell (hPSC) derived pancreatic beta cells are a promising and potentially limitless source for cell replacement therapy. Here the authors perform stage-wise chemical screening to develop an improved protocol for hPSC differentiation to functional pancreatic beta cells at high efficiency.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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