Waffle Method: A general and flexible approach for improving throughput in FIB-milling

Kotaro Kelley; Ashleigh M. Raczkowski; Oleg Klykov; Pattana Jaroenlak; Daija Bobe; Mykhailo Kopylov; Edward T. Eng; Gira Bhabha; Clinton S. Potter; Bridget Carragher; Alex J. Noble

doi:10.1038/s41467-022-29501-3

Nature Communications (Apr 2022)

Waffle Method: A general and flexible approach for improving throughput in FIB-milling

Kotaro Kelley,
Ashleigh M. Raczkowski,
Oleg Klykov,
Pattana Jaroenlak,
Daija Bobe,
Mykhailo Kopylov,
Edward T. Eng,
Gira Bhabha,
Clinton S. Potter,
Bridget Carragher,
Alex J. Noble

Affiliations

Kotaro Kelley: National Center for In-situ Tomographic Ultramicroscopy, Simons Electron Microscopy Center, New York Structural Biology Center
Ashleigh M. Raczkowski: National Center for In-situ Tomographic Ultramicroscopy, Simons Electron Microscopy Center, New York Structural Biology Center
Oleg Klykov: National Center for In-situ Tomographic Ultramicroscopy, Simons Electron Microscopy Center, New York Structural Biology Center
Pattana Jaroenlak: Skirball Institute of Biomolecular Medicine and Department of Cell Biology, New York University School of Medicine
Daija Bobe: National Center for In-situ Tomographic Ultramicroscopy, Simons Electron Microscopy Center, New York Structural Biology Center
Mykhailo Kopylov: National Center for In-situ Tomographic Ultramicroscopy, Simons Electron Microscopy Center, New York Structural Biology Center
Edward T. Eng: National Center for In-situ Tomographic Ultramicroscopy, Simons Electron Microscopy Center, New York Structural Biology Center
Gira Bhabha: Skirball Institute of Biomolecular Medicine and Department of Cell Biology, New York University School of Medicine
Clinton S. Potter: National Center for In-situ Tomographic Ultramicroscopy, Simons Electron Microscopy Center, New York Structural Biology Center
Bridget Carragher: National Center for In-situ Tomographic Ultramicroscopy, Simons Electron Microscopy Center, New York Structural Biology Center
Alex J. Noble: National Center for In-situ Tomographic Ultramicroscopy, Simons Electron Microscopy Center, New York Structural Biology Center

DOI: https://doi.org/10.1038/s41467-022-29501-3
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 13

Abstract

Read online

Here the authors describe the Waffle Method, aimed at increasing the throughput of and solves several challenges present in cryo-FIB/SEM sample preparation for cryo-ET analysis — the highest-resolution method for obtaining 3D views of native biological specimens in-situ.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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