Extracellular nicotinate phosphoribosyltransferase binds Toll like receptor 4 and mediates inflammation

Antonella Managò; Valentina Audrito; Francesca Mazzola; Leonardo Sorci; Federica Gaudino; Katiuscia Gizzi; Nicoletta Vitale; Danny Incarnato; Gabriele Minazzato; Alice Ianniello; Antonio Varriale; Sabato D’Auria; Giulio Mengozzi; Gianfranco Politano; Salvatore Oliviero; Nadia Raffaelli; Silvia Deaglio

doi:10.1038/s41467-019-12055-2

Nature Communications (Sep 2019)

Extracellular nicotinate phosphoribosyltransferase binds Toll like receptor 4 and mediates inflammation

Antonella Managò,
Valentina Audrito,
Francesca Mazzola,
Leonardo Sorci,
Federica Gaudino,
Katiuscia Gizzi,
Nicoletta Vitale,
Danny Incarnato,
Gabriele Minazzato,
Alice Ianniello,
Antonio Varriale,
Sabato D’Auria,
Giulio Mengozzi,
Gianfranco Politano,
Salvatore Oliviero,
Nadia Raffaelli,
Silvia Deaglio

Affiliations

Antonella Managò: Department of Medical Sciences, University of Turin
Valentina Audrito: Department of Medical Sciences, University of Turin
Francesca Mazzola: Department of Clinical Sciences, Polytechnic University of Marche
Leonardo Sorci: Department of Materials, Environmental Sciences and Urban Planning, Division of Bioinformatics and Biochemistry, Polytechnic University of Marche
Federica Gaudino: Department of Medical Sciences, University of Turin
Katiuscia Gizzi: Italian Institute for Genomic Medicine
Nicoletta Vitale: Department of Molecular Biotechnology and Health Sciences, University of Turin
Danny Incarnato: Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen
Gabriele Minazzato: Department of Agricultural, Food and Environmental Sciences, Polytechnic University of Marche
Alice Ianniello: Department of Laboratory Medicine, Azienda Ospedaliero-Universitaria Città della Salute e della Scienza
Antonio Varriale: Institute of Food Science, CNR
Sabato D’Auria: Institute of Food Science, CNR
Giulio Mengozzi: Department of Laboratory Medicine, Azienda Ospedaliero-Universitaria Città della Salute e della Scienza
Gianfranco Politano: Department of Control and Computer Engineering, Polytechnic University of Turin
Salvatore Oliviero: Italian Institute for Genomic Medicine
Nadia Raffaelli: Department of Agricultural, Food and Environmental Sciences, Polytechnic University of Marche
Silvia Deaglio: Department of Medical Sciences, University of Turin

DOI: https://doi.org/10.1038/s41467-019-12055-2
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 14

Abstract

Read online

The enzyme nicotinate phosphoribosyltransferase (NAPRT) mediates the rate-limiting step in NAD salvage pathway starting from nicotinic acid. Here the authors show that NAPRT can be detected extracellularly, binds to Toll like receptor 4, and activates NF-kB signaling and cytokine production in macrophage via NAD synthesis-independent pathways.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal