Nature Communications (May 2018)
Precise control of SCRaMbLE in synthetic haploid and diploid yeast
- Bin Jia,
- Yi Wu,
- Bing-Zhi Li,
- Leslie A. Mitchell,
- Hong Liu,
- Shuo Pan,
- Juan Wang,
- Hao-Ran Zhang,
- Nan Jia,
- Bo Li,
- Michael Shen,
- Ze-Xiong Xie,
- Duo Liu,
- Ying-Xiu Cao,
- Xia Li,
- Xiao Zhou,
- Hao Qi,
- Jef D. Boeke,
- Ying-Jin Yuan
Affiliations
- Bin Jia
- Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University
- Yi Wu
- Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University
- Bing-Zhi Li
- Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University
- Leslie A. Mitchell
- Institute for Systems Genetics, New York University Langone Medical Center
- Hong Liu
- Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University
- Shuo Pan
- Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University
- Juan Wang
- Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University
- Hao-Ran Zhang
- Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University
- Nan Jia
- Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University
- Bo Li
- Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University
- Michael Shen
- Institute for Systems Genetics, New York University Langone Medical Center
- Ze-Xiong Xie
- Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University
- Duo Liu
- Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University
- Ying-Xiu Cao
- Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University
- Xia Li
- Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University
- Xiao Zhou
- Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University
- Hao Qi
- Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University
- Jef D. Boeke
- Institute for Systems Genetics, New York University Langone Medical Center
- Ying-Jin Yuan
- Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University
- DOI
- https://doi.org/10.1038/s41467-018-03084-4
- Journal volume & issue
-
Vol. 9,
no. 1
pp. 1 – 13
Abstract
The SCRaMbLE system integrated into Sc2.0’s synthetic yeast chromosome project allows rapid strain evolution. Here the authors use a genetic logic gate to control induction of recombination in a haploid and diploid yeast carrying synthetic chromosomes.
