Effects of Elaidic Acid on HDL Cholesterol Uptake Capacity

Takuya Iino; Ryuji Toh; Manabu Nagao; Masakazu Shinohara; Amane Harada; Katsuhiro Murakami; Yasuhiro Irino; Makoto Nishimori; Sachiko Yoshikawa; Yutaro Seto; Tatsuro Ishida; Ken-ichi Hirata

doi:10.3390/nu13093112

Nutrients (Sep 2021)

Effects of Elaidic Acid on HDL Cholesterol Uptake Capacity

Takuya Iino,
Ryuji Toh,
Manabu Nagao,
Masakazu Shinohara,
Amane Harada,
Katsuhiro Murakami,
Yasuhiro Irino,
Makoto Nishimori,
Sachiko Yoshikawa,
Yutaro Seto,
Tatsuro Ishida,
Ken-ichi Hirata

Affiliations

Takuya Iino: Division of Cardiovascular Medicine, Graduate School of Medicine, Kobe University, Kobe 650-0017, Japan
Ryuji Toh: Division of Evidence-Based Laboratory Medicine, Graduate School of Medicine, Kobe University, Kobe 650-0017, Japan
Manabu Nagao: Division of Evidence-Based Laboratory Medicine, Graduate School of Medicine, Kobe University, Kobe 650-0017, Japan
Masakazu Shinohara: Division of Epidemiology, Graduate School of Medicine, Kobe University, Kobe 650-0017, Japan
Amane Harada: Central Research Laboratories, Sysmex Corporation, 4-4-4 Takatsukadai, Nishi-ku, Kobe 651-2271, Japan
Katsuhiro Murakami: Central Research Laboratories, Sysmex Corporation, 4-4-4 Takatsukadai, Nishi-ku, Kobe 651-2271, Japan
Yasuhiro Irino: Central Research Laboratories, Sysmex Corporation, 4-4-4 Takatsukadai, Nishi-ku, Kobe 651-2271, Japan
Makoto Nishimori: Division of Cardiovascular Medicine, Graduate School of Medicine, Kobe University, Kobe 650-0017, Japan
Sachiko Yoshikawa: Division of Cardiovascular Medicine, Graduate School of Medicine, Kobe University, Kobe 650-0017, Japan
Yutaro Seto: Division of Cardiovascular Medicine, Graduate School of Medicine, Kobe University, Kobe 650-0017, Japan
Tatsuro Ishida: Division of Cardiovascular Medicine, Graduate School of Medicine, Kobe University, Kobe 650-0017, Japan
Ken-ichi Hirata: Division of Cardiovascular Medicine, Graduate School of Medicine, Kobe University, Kobe 650-0017, Japan

DOI: https://doi.org/10.3390/nu13093112
Journal volume & issue: Vol. 13, no. 9
p. 3112

Abstract

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Recently we established a cell-free assay to evaluate “cholesterol uptake capacity (CUC)” as a novel concept for high-density lipoprotein (HDL) functionality and demonstrated the feasibility of CUC for coronary risk stratification, although its regulatory mechanism remains unclear. HDL fluidity affects cholesterol efflux, and trans fatty acids (TFA) reduce lipid membrane fluidity when incorporated into phospholipids (PL). This study aimed to clarify the effect of TFA in HDL-PL on CUC. Serum was collected from 264 patients after coronary angiography or percutaneous coronary intervention to measure CUC and elaidic acid levels in HDL-PL, and in vitro analysis using reconstituted HDL (rHDL) was used to determine the HDL-PL mechanism affecting CUC. CUC was positively associated with HDL-PL levels but negatively associated with the proportion of elaidic acid in HDL-PL (elaidic acid in HDL-PL/HDL-PL ratio). Increased elaidic acid-phosphatidylcholine (PC) content in rHDL exhibited no change in particle size or CUC compared to rHDL containing oleic acid in PC. Recombinant human lecithin-cholesterol acyltransferase (LCAT) enhanced CUC, and LCAT-dependent enhancement of CUC and LCAT-dependent cholesterol esterification were suppressed in rHDL containing elaidic acid in PC. Therefore, CUC is affected by HDL-PL concentration, HDL-PL acyl group composition, and LCAT-dependent cholesterol esterification. Elaidic acid precipitated an inhibition of cholesterol uptake and maturation of HDL; therefore, modulation of HDL-PL acyl groups could improve CUC.

Published in Nutrients

ISSN: 2072-6643 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.mdpi.com/journal/nutrients

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