Data in support for the measurement of heparan sulfate and dermatan sulfate by LC–MS/MS analysis

Giulia Forni; Sabrina Malvagia; Silvia Funghini; Emanuela Scolamiero; Massimo Mura; Maria Della Bona; Fabio Villanelli; Roberta Damiano; Giancarlo la Marca

Data in Brief (Dec 2018)

Data in support for the measurement of heparan sulfate and dermatan sulfate by LC–MS/MS analysis

Giulia Forni,
Sabrina Malvagia,
Silvia Funghini,
Emanuela Scolamiero,
Massimo Mura,
Maria Della Bona,
Fabio Villanelli,
Roberta Damiano,
Giancarlo la Marca

Affiliations

Giulia Forni: Newborn Screening, Biochemistry and Pharmacology Laboratory, Pediatric Neurology Unit and Laboratories, Meyer Children׳s University Hospital, Florence, Italy
Sabrina Malvagia: Newborn Screening, Biochemistry and Pharmacology Laboratory, Pediatric Neurology Unit and Laboratories, Meyer Children׳s University Hospital, Florence, Italy
Silvia Funghini: Newborn Screening, Biochemistry and Pharmacology Laboratory, Pediatric Neurology Unit and Laboratories, Meyer Children׳s University Hospital, Florence, Italy
Emanuela Scolamiero: Newborn Screening, Biochemistry and Pharmacology Laboratory, Pediatric Neurology Unit and Laboratories, Meyer Children׳s University Hospital, Florence, Italy; Department of Experimental and Clinical Biomedical Sciences, University of Florence, Florence, Italy
Massimo Mura: Newborn Screening, Biochemistry and Pharmacology Laboratory, Pediatric Neurology Unit and Laboratories, Meyer Children׳s University Hospital, Florence, Italy
Maria Della Bona: Newborn Screening, Biochemistry and Pharmacology Laboratory, Pediatric Neurology Unit and Laboratories, Meyer Children׳s University Hospital, Florence, Italy
Fabio Villanelli: Newborn Screening, Biochemistry and Pharmacology Laboratory, Pediatric Neurology Unit and Laboratories, Meyer Children׳s University Hospital, Florence, Italy
Roberta Damiano: Newborn Screening, Biochemistry and Pharmacology Laboratory, Pediatric Neurology Unit and Laboratories, Meyer Children׳s University Hospital, Florence, Italy; Department of Experimental and Clinical Biomedical Sciences, University of Florence, Florence, Italy
Giancarlo la Marca: Newborn Screening, Biochemistry and Pharmacology Laboratory, Pediatric Neurology Unit and Laboratories, Meyer Children׳s University Hospital, Florence, Italy; Department of Experimental and Clinical Biomedical Sciences, University of Florence, Florence, Italy; Corresponding author at: Newborn Screening, Biochemistry and Pharmacology Laboratory, Pediatric Neurology Unit and Laboratories, Meyer Children׳s University Hospital, Florence, Italy.

Journal volume & issue: Vol. 21
pp. 2398 – 2404

Abstract

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This article provides supplementary data for the paper “LC–MS/MS method for simultaneous quantification of heparan sulfate and dermatan sulfate in urine by butanolysis derivatization” (Forni et al., 2018). Several parameters were tested to optimize sample preparation by butanolysis in order to carry out simultaneous quantifications of HS and DS by tandem mass spectrometry.Here we describe step-by-step instructions to perform HS and DS analysis in urine samples using external calibration curves of standards of known concentration. Sample are quantified by interpolation from the calibration curve and reported in µg/mL. Then, HS and DS are normalized to creatinine concentration and reported as mg/g uCr.

Published in Data in Brief

ISSN: 2352-3409 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Medicine: Medicine (General): Computer applications to medicine. Medical informatics; Science: Science (General)
Website: http://www.journals.elsevier.com/data-in-brief/

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