Biossurfactant production by isolated bacteria from petroleum industry’s effluent targeting bioremediation

Katiany do Vale Vale; Darlane Wellen Freitas Soares; Ana Luiza Beserra da Silva; Flavia Oliveira Monteiro da Silva Abreu; João Lucas Isidio de Oliveira Almeida; Maria Roniele Félix Oliveira; Liange Reck; Vânia Maria Maciel Melo; Daniela Ribeiro Alves; Carlucio Roberto Alves

Results in Chemistry (Dec 2023)

Biossurfactant production by isolated bacteria from petroleum industry’s effluent targeting bioremediation

Katiany do Vale Vale,
Darlane Wellen Freitas Soares,
Ana Luiza Beserra da Silva,
Flavia Oliveira Monteiro da Silva Abreu,
João Lucas Isidio de Oliveira Almeida,
Maria Roniele Félix Oliveira,
Liange Reck,
Vânia Maria Maciel Melo,
Daniela Ribeiro Alves,
Carlucio Roberto Alves

Affiliations

Katiany do Vale Vale: Laboratory Systems in Nanotechnology and Biomaterials, Postgraduate Program in Biotechnology, Science and Technology Center – State University of Ceará, Avenida Dr. Silas Munguba, 1700 – CEP: 60.714 – 903 – Campus Itaperi, Fortaleza, CE, Brazil
Darlane Wellen Freitas Soares: Laboratory of Chemical Processes, Professor of the Undergraduate Course in Chemical Engineering – University Center UniFanor Wyden, Rua Antônio Gomes Guimarães, 150 – Papicu, CEP: 60191-195 – Campus Dunas, Fortaleza, CE, Brazil
Ana Luiza Beserra da Silva: Laboratory Systems in Nanotechnology and Biomaterials, Postgraduate Program in Natural Sciences, Science and Technology Center – State University of Ceará, Avenida Dr. Silas Munguba, 1700 – CEP: 60.714 – 903 – Campus Itaperi, Fortaleza, CE, Brazil
Flavia Oliveira Monteiro da Silva Abreu: Laboratory of Natural Polymers, Postgraduate Program in Natural Sciences, Science and Technology Center – State University of Ceará, Avenida Dr. Silas Munguba, 1700 – CEP: 60.714 – 903 – Campus Itaperi, Fortaleza, CE, Brazil; Corresponding author.
João Lucas Isidio de Oliveira Almeida: Laboratory of Natural Polymers, Postgraduate Program in Natural Sciences, Science and Technology Center – State University of Ceará, Avenida Dr. Silas Munguba, 1700 – CEP: 60.714 – 903 – Campus Itaperi, Fortaleza, CE, Brazil
Maria Roniele Félix Oliveira: Laboratory Systems in Nanotechnology and Biomaterials, Postgraduate Program in Biotechnology, Science and Technology Center – State University of Ceará, Avenida Dr. Silas Munguba, 1700 – CEP: 60.714 – 903 – Campus Itaperi, Fortaleza, CE, Brazil
Liange Reck: Laboratory Systems in Nanotechnology and Biomaterials, Postgraduate Program in Biotechnology, Science and Technology Center – State University of Ceará, Avenida Dr. Silas Munguba, 1700 – CEP: 60.714 – 903 – Campus Itaperi, Fortaleza, CE, Brazil
Vânia Maria Maciel Melo: Laboratory of Microbial Ecology and Biotechnology, Postgraduate Program in Biotechnology, Science Center, Department of Biology – Federal University of Ceará, Pici Bloco 909 – CEP: 60455-760 – Campus do Pici, Fortaleza, CE, Brazil
Daniela Ribeiro Alves: Laboratory of Chemistry of Natural Products, Postgraduate Program in Natural Sciences, Science and Technology Center, State University of Ceará, Avenida Dr. Silas Munguba, 1700 – CEP: 60.714 – 903 – Campus Itaperi, Fortaleza, CE, Brazil
Carlucio Roberto Alves: Laboratory Systems in Nanotechnology and Biomaterials, Postgraduate Program in Biotechnology, Science and Technology Center – State University of Ceará, Avenida Dr. Silas Munguba, 1700 – CEP: 60.714 – 903 – Campus Itaperi, Fortaleza, CE, Brazil

Journal volume & issue: Vol. 6
p. 101038

Abstract

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In this study, bacterial strains from the Bacillus genus were isolated from residual effluents from treatment unit of a Petroleum Factory to produce a biosurfactant, aiming the bioremediation of oil-contaminated soils. Five strains were selected to perform fermentation tests. Results showed that the strain LUB P1 presented a lipopeptide with excellent properties, which reduced the surface tension to rates below 30 mN/m. The bacteria were genetically identified as a species belonging to the Bacillus subtilis. Characterization of the biosurfactant confirmed that the main component is surfactin, with a critical micellar concentration (CMC) value of 15 mg.L−1 and high stability to temperature, salinity, and pH variations. In addition, showed low toxicity compared to synthetic surfactants. Bioremediation tests indicated motor oil removal efficiency of over 80% on the temperature of 60 °C in its CMC, presenting excellent properties for the application in bioremediation.

Published in Results in Chemistry

ISSN: 2211-7156 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Chemistry
Website: https://www.journals.elsevier.com/results-in-chemistry/

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