Iranian Journal of Chemistry & Chemical Engineering (Jun 2013)

Bisubstrate Kinetic Model for Enzymatic Decolorization of Reactive Black 5 by Coprinus cinereus Peroxidase

  • Mohammad Mansouri Majoumerd,
  • Hamid Reza Kariminia

Journal volume & issue
Vol. 32, no. 2
pp. 125 – 134

Abstract

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In this study, decolorization of the diazo dye, Reactive Black 5 (RB5) in a Coprinus cinereus peroxidase-catalyzed reaction has been investigated. A bisubstate kinetic model for the reaction rate based on the Ping-Pong mechanism was assumed for the enzymatic decolorization. Experimentswere conducted at different RB5 and hydrogen peroxideconcentrations in a batch mannerto estimate the intrinsic kinetic parameters. These parameters were used for the modeling of decolorization in a continuous reactor that was compared with experimental results. An acceptable agreement was observed between the model and experimental data.

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