MONOLITHIC DISK FOR THE FAST CHROMATOGRAPHIC SEPARATION

Nurul Hidayat Aprilita; Rania Bakry; Christian W Huck; Guenther K Bonn

doi:10.22146/ijc.21815

Indonesian Journal of Chemistry (Jun 2010)

MONOLITHIC DISK FOR THE FAST CHROMATOGRAPHIC SEPARATION

Nurul Hidayat Aprilita,
Rania Bakry,
Christian W Huck,
Guenther K Bonn

Affiliations

Nurul Hidayat Aprilita: Chemistry Department, Faculty of Mathematics and Natural Sciences, Gadjah Mada University,
Rania Bakry: Institute of Analytical Chemistry and Radiochemistry, University of Innsbruck,
Christian W Huck: Institute of Analytical Chemistry and Radiochemistry, University of Innsbruck,
Guenther K Bonn: Institute of Analytical Chemistry and Radiochemistry, University of Innsbruck,

DOI: https://doi.org/10.22146/ijc.21815
Journal volume & issue: Vol. 5, no. 2
pp. 108 – 114

Abstract

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Poly(styrene/divinylbenzene) (PS/DVB) monolithic disk was prepared by in situ free-radical copolymerization of styrene and divinylbenzene in the presence of decanol and tetrahydrofuran as porogens. PS/DVB monolithic disks were produced in two different lengths 1.5 mm and 3 mm. The disks were used in reversed phase chromatography of proteins with 0.2 % trifuoroacetic acid (TFA) and 0.2 % TFA in acetonitrile as mobile phase A and B, respectively. The effect of gradient rate, flow rate, temperature and disk length on the separation of proteins were also studied. PS/DVB monolithic disks allow the rapid separation of proteins in reversed phase chromatography. Keywords: monolithic disk, poly(styrene/divinylbenzene), proteins

Published in Indonesian Journal of Chemistry

ISSN: 1411-9420 (Print); 2460-1578 (Online)
Publisher: Department of Chemistry, Universitas Gadjah Mada
Country of publisher: Indonesia
LCC subjects: Science: Chemistry
Website: https://jurnal.ugm.ac.id/ijc/

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