Detection of biological toxicity of haze with algae fluorescence

Abstract

The comprehensive toxicity of haze cannot be evaluated by evaluating each pollutant separately because haze contains complex components. In order to quickly, accurately and comprehensively evaluate the comprehensive toxicity of haze, Algae chlorophyll fluorescence method is used to assess the biological toxicity. With Chlorella pyrenoidosa and Microcystis aeruginosa used as experiment algae, and the haze in the heating period as the research object, the solution absorption method and membrane weighing method are used to collect the liquid absorption haze and total suspended particles haze (TSP), respectively. The change of fluorescence parameters (Y(Ⅱ), NPQ, qP, ETR, Fv/Fm) of single algae under the stress of the haze absorption liquid and the total suspended particulate matter are detected by water fluorescence detector. The results show that Chlorella pyrenoidosa is more sensitive to exogenous haze exposure, and the fluorescence parameters decreases significantly, especially that the concentration of NPQ decreases by 46.3% and 40.2% under the influence of haze absorption in the two days of heavy pollution, while Microcystis aeruginosa displays obvious characterization only in the interference of total suspended particulate matter leaching solution. Therefore, it is possible that the NPQ parameter of Chlorella pyrenoidosa is selected as an indicator for haze biological toxicity.

Keywords

• environmental toxicology
• biological toxicity
• algae fluorescence
• haze
• Chlorella pyrenoidosa
• Microcystis aeruginosa