Cold Atmospheric Pressure Plasma Is Effective against <i>P. gingivalis</i> (HW24D-1) Mature Biofilms and Non-Genotoxic to Oral Cells

Abstract

The effects of helium cold atmospheric pressure plasma (He-CAPP) jet on Porphyromonas gingivalis (HW24D-1) biofilm, on human gingival fibroblasts (HGF) and human gingival keratinocytes (OBA-9) were assessed. Standardized suspension of P. gingivalis was obtained, and biofilms were grown anaerobically for 48 h. After exposition to He-CAPP, the biofilm viability was evaluated by XTT assay. HGF were grown at 37 °C, in an CO2 chamber in DMEM, while OBA-9 cells were cultured in keratinocyte serum-free medium. After 24 h, plates were exposed to He-CAPP for 1 to 7 min. Plasma was generated using a commercial AC power supply with amplitude modulated signal (voltage amplitude of 20 kVp-p, frequency of 31.0 kHz and duty cycle of 22%). The corresponding discharge power was 0.6W at He flow rate of 1 L/min. DNA damage was accessed by static cytometry. Data were analyzed by GraphPad Prism (p P. gingivalis viability in relation to non-treated groups were detected (p P. gingivalis mature biofilm and was not genotoxic for epithelial gingival cells and human oral fibroblasts.

Keywords

• cold plasma
• periodontitis
• <i>Porphyromonas gingivalis</i>
• genetic toxicity test