Single-exonuclease nanocircuits reveal the RNA degradation dynamics of PNPase and demonstrate potential for RNA sequencing

Zhiheng Yang; Wenzhe Liu; Lihua Zhao; Dongbao Yin; Jianfei Feng; Lidong Li; Xuefeng Guo

doi:10.1038/s41467-023-36278-6

Nature Communications (Feb 2023)

Single-exonuclease nanocircuits reveal the RNA degradation dynamics of PNPase and demonstrate potential for RNA sequencing

Zhiheng Yang,
Wenzhe Liu,
Lihua Zhao,
Dongbao Yin,
Jianfei Feng,
Lidong Li,
Xuefeng Guo

Affiliations

Zhiheng Yang: State Key Laboratory for Advanced Metals and Materials, School of Materials Science and Engineering, University of Science and Technology Beijing
Wenzhe Liu: Beijing National Laboratory for Molecular Sciences, National Biomedical Imaging Centre, College of Chemistry and Molecular Engineering, Peking University, 292 Chengfu Road, Haidian District
Lihua Zhao: Beijing National Laboratory for Molecular Sciences, National Biomedical Imaging Centre, College of Chemistry and Molecular Engineering, Peking University, 292 Chengfu Road, Haidian District
Dongbao Yin: State Key Laboratory for Advanced Metals and Materials, School of Materials Science and Engineering, University of Science and Technology Beijing
Jianfei Feng: Beijing National Laboratory for Molecular Sciences, National Biomedical Imaging Centre, College of Chemistry and Molecular Engineering, Peking University, 292 Chengfu Road, Haidian District
Lidong Li: State Key Laboratory for Advanced Metals and Materials, School of Materials Science and Engineering, University of Science and Technology Beijing
Xuefeng Guo: Beijing National Laboratory for Molecular Sciences, National Biomedical Imaging Centre, College of Chemistry and Molecular Engineering, Peking University, 292 Chengfu Road, Haidian District

DOI: https://doi.org/10.1038/s41467-023-36278-6
Journal volume & issue: Vol. 14, no. 1
pp. 1 – 14

Abstract

Read online

Observing the natural process of RNA degradation in real-time is a significant challenge. Here, the authors develop and use single-exonuclease nanocircuits to reveal the single-base degradation behaviour of PNPase, and demonstrate proof-of-principle RNA sequencing using this approach.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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