Generation of a TLE1 homozygous knockout human embryonic stem cell line using CRISPR-Cas9

Amanda Herring; Angelica Messana; Anne M. Bara; Dane Z. Hazelbaker; Kevin Eggan; Lindy E. Barrett

doi:10.1016/j.scr.2016.09.009

Stem Cell Research (Sep 2016)

Generation of a TLE1 homozygous knockout human embryonic stem cell line using CRISPR-Cas9

Amanda Herring,
Angelica Messana,
Anne M. Bara,
Dane Z. Hazelbaker,
Kevin Eggan,
Lindy E. Barrett

Affiliations

Amanda Herring: Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA
Angelica Messana: Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA
Anne M. Bara: Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA
Dane Z. Hazelbaker: Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA
Kevin Eggan: Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA
Lindy E. Barrett: Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA

DOI: https://doi.org/10.1016/j.scr.2016.09.009
Journal volume & issue: Vol. 17, no. 2
pp. 430 – 432

Abstract

Read online

Here, we generated a biallelic mutation in the TLE1 (Transducin Like Enhancer of Split 1) gene using CRISPR-Cas9 editing in the human embryonic stem cell (hESC) line WA01. The homozygous knockout cell line, TLE1-464-G04, displays loss of TLE1 protein expression while maintaining pluripotency, differentiation potential and genomic integrity.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

About the journal