Functional Analysis of a Wheat AGPase Plastidial Small Subunit with a Truncated Transit Peptide

Yang Yang; Tian Gao; Mengjun Xu; Jie Dong; Hanxiao Li; Pengfei Wang; Gezi Li; Tiancai Guo; Guozhang Kang; Yonghua Wang

doi:10.3390/molecules22030386

Molecules (Mar 2017)

Functional Analysis of a Wheat AGPase Plastidial Small Subunit with a Truncated Transit Peptide

Yang Yang,
Tian Gao,
Mengjun Xu,
Jie Dong,
Hanxiao Li,
Pengfei Wang,
Gezi Li,
Tiancai Guo,
Guozhang Kang,
Yonghua Wang

Affiliations

Yang Yang: The Collaborative Innovation Center of Henan Food Crops, Henan Agricultural University, Zhengzhou 450002, China
Tian Gao: The Collaborative Innovation Center of Henan Food Crops, Henan Agricultural University, Zhengzhou 450002, China
Mengjun Xu: The National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou 450002, China
Jie Dong: The National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou 450002, China
Hanxiao Li: The National Engineering Research Centre for Wheat, Henan Agricultural University, Zhengzhou 450002, China
Pengfei Wang: The Collaborative Innovation Center of Henan Food Crops, Henan Agricultural University, Zhengzhou 450002, China
Gezi Li: The National Engineering Research Centre for Wheat, Henan Agricultural University, Zhengzhou 450002, China
Tiancai Guo: The National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou 450002, China
Guozhang Kang: The National Engineering Research Centre for Wheat, Henan Agricultural University, Zhengzhou 450002, China
Yonghua Wang: The Collaborative Innovation Center of Henan Food Crops, Henan Agricultural University, Zhengzhou 450002, China

DOI: https://doi.org/10.3390/molecules22030386
Journal volume & issue: Vol. 22, no. 3
p. 386

Abstract

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ADP-glucose pyrophosphorylase (AGPase), the key enzyme in starch synthesis, consists of two small subunits and two large subunits with cytosolic and plastidial isoforms. In our previous study, a cDNA sequence encoding the plastidial small subunit (TaAGPS1b) of AGPase in grains of bread wheat (Triticum aestivum L.) was isolated and the protein subunit encoded by this gene was characterized as a truncated transit peptide (about 50% shorter than those of other plant AGPS1bs). In the present study, TaAGPS1b was fused with green fluorescent protein (GFP) in rice protoplast cells, and confocal fluorescence microscopy observations revealed that like other AGPS1b containing the normal transit peptide, TaAGPS1b-GFP was localized in chloroplasts. TaAGPS1b was further overexpressed in a Chinese bread wheat cultivar, and the transgenic wheat lines exhibited a significant increase in endosperm AGPase activities, starch contents, and grain weights. These suggested that TaAGPS1b subunit was targeted into plastids by its truncated transit peptide and it could play an important role in starch synthesis in bread wheat grains.

Published in Molecules

ISSN: 1420-3049 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Chemistry: Organic chemistry
Website: http://www.mdpi.com/journal/molecules

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