Association of Gln27Glu Polymorphism of the β2-adrenoreceptor Gene with Specific Parameters of the Immunoregulatory Cytokine Cascade and IgE Production in Patients with Bronchial Asthma

Abstract

The objective: to study the level of serum cytokines IL-4, IFN-γ and the activity of IgE production in patients with bronchial asthma (BA) with the consideration of the Gln27Glu-polymorphism of the β2-AR gene.Subjects and Methods. 130 people of Uzbek ethnicity were examined including 83 patients with BA. BA patients were divided into groups according to the international classification of the World Health Organization and the GINA 2006 diagnostic criteria. Genotyping was performed by polymerase chain reaction technology and restriction fragment analysis.Results. In the group with allergic BA, patients with the Gln27Gln-polymorphic variant of the β2-adrenergic receptor gene had an average IgE value (1,664.20 ± 293.29 pg/ml) higher versus patients with the Gln27Glu genotype (338.40 ± 121.33 pg/ml; p < 0.01). No significant relationship between the Gln27Glu polymorphism of the β2-adrenergic receptor gene and the activity of inductor cytokines was found. The Gln27Gln genotype of the β2-adrenergic receptor gene in patients with bronchial asthma, especially in the allergic form of the disease, is associated with hyperproduction of IgE.

Keywords

• бронхиальная астма
• gln27glu-полиморфизм гена β2-адренорецептора
• цитокины il-4
• ifn-γ
• ige