In Vitro Evaluation of Cytotoxicity and Anticancer Activity of Herbal Extract V2S2 (Coded Drug) on Human Cancer Cell Lines

Abstract

Background Cancer is the leading cause of morbidity and mortality worldwide. It encompasses a large family of diseases exhibiting abnormal cell growth with the potential to invade or spread to other part of the body. Natural extracts have been used as an efficient anticancer agent that show promising results. In this, study the cytotoxicity and anticancer effect of Ayurvedic herbal extract code name V2S2 was studied and compared with control drug Adriamycin that is an established chemotherapeutic drug. Materials and Methods Ayurvedic coded drug V2S2 is a hydroalcoholic extract of herbs. The in vitro anticancer study was performed with sulforhodamine B assay in TATA Memorial, ACTREC Mumbai as per National Cancer Institute guidelines. Result GI50 of V2S2 study drug and Adriamycin on COLO-205, HOP-62, K-562, ISHIKAWA, HeLa, MCF-7, SCC-40, Hep-G2 and MIA-PA-CA-2 is less than 10 that means both drugs have equal anticancer activity. Conclusion The study group has more and less equal potential of anticancer activity in compared with a transient's standard anticancer drug. Moreover, in vitro monkey kidney normal cell line study drug V2S2 shows same cytotoxicity as shown by control drug Adriamycin.

Keywords

• cancer
• gi50
• v2s2
• adriamycin