Journal of Immunology Research (Jan 2020)
Genes Induced by Panax Notoginseng in a Rodent Model of Ischemia-Reperfusion Injury
- Lanqing Meng,
- Qing Huang,
- Xuebin Li,
- Ping Liang,
- Yueyong Li,
- Xiaohua Huang,
- Jingjie Zhao,
- Qiuping Chen,
- Rong Qiu,
- Lan Li,
- Chongdong Jian,
- Hongfei Yao,
- Jianmin Huang,
- Xionglin Tang,
- Zechen Wang,
- Zhongheng Wei,
- Jun Wu,
- Liuzhi Wei,
- Qiuju Wei,
- Qianli Tang,
- Lu Huang,
- Jihua Wei,
- Dinggui Lu,
- Qunqiang Luo,
- Kegong Xie,
- Yang Ouyang,
- Jian Chen,
- Genliang Li,
- Linxue Luo,
- Linbo He,
- Chenyi Zhuo,
- Anding Xu,
- Lingzhang Meng
Affiliations
- Lanqing Meng
- Jinan University, Guangzhou City, Guangdong Province, China
- Qing Huang
- Department of Neurology, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise City, Guangxi Province, China
- Xuebin Li
- Department of Neurology, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise City, Guangxi Province, China
- Ping Liang
- Department of Neurology, The First People’s Hospital of Yulin, Yulin City, Guangxi Province, China
- Yueyong Li
- Department of Inverventional Medicine, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise City, Guangxi Province, China
- Xiaohua Huang
- Jinan University, Guangzhou City, Guangdong Province, China
- Jingjie Zhao
- Life Science and Clinical Research Center, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise City, Guangxi Province, China
- Qiuping Chen
- Department of Rehabilitation Medicine, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise City, Guangxi Province, China
- Rong Qiu
- Graduate School of Youjiang Medical University for Nationalities, Baise City, Guangxi Province, China
- Lan Li
- Graduate School of Youjiang Medical University for Nationalities, Baise City, Guangxi Province, China
- Chongdong Jian
- Department of Neurology, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise City, Guangxi Province, China
- Hongfei Yao
- Graduate School of Youjiang Medical University for Nationalities, Baise City, Guangxi Province, China
- Jianmin Huang
- Department of Neurology, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise City, Guangxi Province, China
- Xionglin Tang
- Department of Neurology, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise City, Guangxi Province, China
- Zechen Wang
- Graduate School of Youjiang Medical University for Nationalities, Baise City, Guangxi Province, China
- Zhongheng Wei
- Department of Inverventional Medicine, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise City, Guangxi Province, China
- Jun Wu
- Urinary Surgery Department, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise City, Guangxi Province, China
- Liuzhi Wei
- Center for Systemic Inflammation Research (CSIR), School of Preclinical Medicine, Youjiang Medical University for Nationalities, Baise City, Guangxi Province, China
- Qiuju Wei
- Center for Systemic Inflammation Research (CSIR), School of Preclinical Medicine, Youjiang Medical University for Nationalities, Baise City, Guangxi Province, China
- Qianli Tang
- Center for Systemic Inflammation Research (CSIR), School of Preclinical Medicine, Youjiang Medical University for Nationalities, Baise City, Guangxi Province, China
- Lu Huang
- Graduate School of Youjiang Medical University for Nationalities, Baise City, Guangxi Province, China
- Jihua Wei
- Trauma Center, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise City, Guangxi Province, China
- Dinggui Lu
- Trauma Center, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise City, Guangxi Province, China
- Qunqiang Luo
- Trauma Center, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise City, Guangxi Province, China
- Kegong Xie
- Trauma Center, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise City, Guangxi Province, China
- Yang Ouyang
- Department of Neurology, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise City, Guangxi Province, China
- Jian Chen
- Graduate School of Youjiang Medical University for Nationalities, Baise City, Guangxi Province, China
- Genliang Li
- Department of Biochemistry and Molecular Biology, Youjiang Medical University for Nationalities, Baise City, Guangxi Province, China
- Linxue Luo
- Department of Gynaecology and Obstetrics, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise City, Guangxi Province, China
- Linbo He
- Shangsi People's Hospital, Fangchenggang City, Guangxi Province, China
- Chenyi Zhuo
- Department of Hepatological Surgery, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise City, Guangxi Province, China
- Anding Xu
- Jinan University, Guangzhou City, Guangdong Province, China
- Lingzhang Meng
- Center for Systemic Inflammation Research (CSIR), School of Preclinical Medicine, Youjiang Medical University for Nationalities, Baise City, Guangxi Province, China
- DOI
- https://doi.org/10.1155/2020/8873261
- Journal volume & issue
-
Vol. 2020
Abstract
Stroke is a cerebrovascular disease that results in decreased blood flow. Although Panax notoginseng (PN), a Chinese herbal medicine, has been proven to promote stroke recovery, its molecular mechanism remains unclear. In this study, middle cerebral artery occlusion (MCAO) was induced in rats with thrombi generated by thread and subsequently treated with PN. After that, staining with 2,3,5-triphenyltetrazolium chloride was employed to evaluate the infarcted area, and electron microscopy was used to assess ultrastructural changes of the neurovascular unit. RNA-Seq was performed to determine the differential expressed genes (DEGs) which were then verified by qPCR. In total, 817 DEGs were identified to be related to the therapeutic effect of PN on stroke recovery. Further analysis by Gene Oncology analysis and Kyoto Encyclopedia of Genes and Genomes revealed that most of these genes were involved in the biological function of nerves and blood vessels through the regulation of neuroactive live receptor interactions of PI3K-Akt, Rap1, cAMP, and cGMP-PKG signaling, which included in the 18 pathways identified in our research, of which, 9 were reported firstly that related to PN’s neuroprotective effect. This research sheds light on the potential molecular mechanisms underlying the effects of PN on stroke recovery.
