The first reported generation of footprint-free human induced pluripotent stem cell line (SCIKFi001-A) in Indonesia from cGMP grade umbilical cord-derived mesenchymal stem cells (UC-MSCs) using synthetic self-replicating RNA

Ricky Sanjaya; Siska Yuliana Sari; Andrian Ibnu Faisal; Matheus Alvin Prawira; Naufalia Faza; Halida P. Widyastuti; Harry Murti

Stem Cell Research (Jul 2022)

The first reported generation of footprint-free human induced pluripotent stem cell line (SCIKFi001-A) in Indonesia from cGMP grade umbilical cord-derived mesenchymal stem cells (UC-MSCs) using synthetic self-replicating RNA

Ricky Sanjaya,
Siska Yuliana Sari,
Andrian Ibnu Faisal,
Matheus Alvin Prawira,
Naufalia Faza,
Halida P. Widyastuti,
Harry Murti

Affiliations

Ricky Sanjaya: Department of Translational Research Stem Cell, Stem Cell and Cancer Institute, PT. Kalbe Farma Tbk., Jakarta, Indonesia; ReGeniC Laboratory, PT. Bifarma Adiluhung, Jakarta, Indonesia
Siska Yuliana Sari: Department of Translational Research Stem Cell, Stem Cell and Cancer Institute, PT. Kalbe Farma Tbk., Jakarta, Indonesia; ReGeniC Laboratory, PT. Bifarma Adiluhung, Jakarta, Indonesia
Andrian Ibnu Faisal: Department of Translational Research Stem Cell, Stem Cell and Cancer Institute, PT. Kalbe Farma Tbk., Jakarta, Indonesia; ReGeniC Laboratory, PT. Bifarma Adiluhung, Jakarta, Indonesia
Matheus Alvin Prawira: Department of Translational Research Stem Cell, Stem Cell and Cancer Institute, PT. Kalbe Farma Tbk., Jakarta, Indonesia; ReGeniC Laboratory, PT. Bifarma Adiluhung, Jakarta, Indonesia
Naufalia Faza: Department of Translational Research Stem Cell, Stem Cell and Cancer Institute, PT. Kalbe Farma Tbk., Jakarta, Indonesia; ReGeniC Laboratory, PT. Bifarma Adiluhung, Jakarta, Indonesia
Halida P. Widyastuti: Department of Translational Research Stem Cell, Stem Cell and Cancer Institute, PT. Kalbe Farma Tbk., Jakarta, Indonesia; ReGeniC Laboratory, PT. Bifarma Adiluhung, Jakarta, Indonesia; Corresponding authors at: Department of Translational Research Stem Cell, Stem Cell and Cancer Institute, PT. Kalbe Farma Tbk., Jakarta, Indonesia.
Harry Murti: Department of Translational Research Stem Cell, Stem Cell and Cancer Institute, PT. Kalbe Farma Tbk., Jakarta, Indonesia; ReGeniC Laboratory, PT. Bifarma Adiluhung, Jakarta, Indonesia; Corresponding authors at: Department of Translational Research Stem Cell, Stem Cell and Cancer Institute, PT. Kalbe Farma Tbk., Jakarta, Indonesia.

Journal volume & issue: Vol. 62
p. 102790

Abstract

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Induced pluripotent stem cell (iPSC) line SCIKFi001-A was reprogrammed from cGMP grade umbilical cord derived mesenchymal stem cells (UC-MSCs) via non-integrating, virus free, self-replicating RNA for eventual use in regenerative medicine. UC-MSCs, a type of multipotent stem cells with fibroblast-like phenotypes, were previously isolated, cryobanked, expanded and characterized in accordance with cGMP principles. The iPSCs generated from this cGMP grade cell line were then characterized and pluripotency was established. Here we showed that UC-MSCs can be reprogrammed to iPSCs using a safer and more regulatory friendly method, which will enable researchers to accelerate their clinical development timeline.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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