Evaluación de actividades enzimáticas de Fusarium spp., aislados de lesiones en humanos, animales y plantas

Abstract

Assessment of enzymatic characterization of Fusarium spp. isolated from human, animal, and plant wounds. Objective. Todetermine amylolytic, cellulolytic, lipolytic, pectinolytic and proteolytic activities in 32 Fusarium spp. isolates from humans, animals andplants. Materials and methods. Qualitative determination of enzymatic activities was done by measuring hydrolysis halos in agar plateswith their corresponding substrate. Quantitative determination was done by colorimetric techniques, using liquid culture supernatantsto determine the respective substrate degradation. Results. All isolates showed enzymatic activities from a qualitative point of view,except amylolytic and lipolytic. Quantitative determination was possible for all the evaluated enzymes except lipases. Conclusion.The determination of amylolytic, cellulolytic, pectinolytic and proteolytic enzymatic profiles of each of the Fusarium isolates assessed suggests their capacity to degrade these substrates, irrespectively of their origin.

Keywords

• Fusarium
• multihost pathogen
• amylases
• celluloses
• pectinases
• proteases
• lipases