Nature Communications (Oct 2016)

A distinct holoenzyme organization for two-subunit pyruvate carboxylase

  • Philip H. Choi,
  • Jeanyoung Jo,
  • Yu-Cheng Lin,
  • Min-Han Lin,
  • Chi-Yuan Chou,
  • Lars E. P. Dietrich,
  • Liang Tong

DOI
https://doi.org/10.1038/ncomms12713
Journal volume & issue
Vol. 7, no. 1
pp. 1 – 13

Abstract

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Pyruvate carboxylases are homotetrameric enzymes in eukaryotes and most bacteria. Here, the authors report the structure of an unusual two-subunit form of the enzyme from the Gram-negative bacterium Methylobacillus flagellates, revealing an unexpected α2β4stoichiometry.