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Comparison of the effects of triamcinolone acetonide or platelet-rich plasma on expression of extracellular matrix-related genes in equine healthy chondrocytes in vitro

  • Heloisa Einloft Palma,
  • Miguel Gallio,
  • Gabriele Biavaschi da Silva,
  • Camila Cantarelli,
  • Kalyne Bertolin,
  • Patrícia Wolkmer,
  • Julien Wergutz,
  • Luciana Maria Fontanari Krause,
  • Alexandre Krause,
  • Alfredo Quites Antoniazzi,
  • Karin Erica Brass,
  • Flavio Desessards De La Corte

DOI
https://doi.org/10.1590/0103-8478cr20180262
Journal volume & issue
Vol. 49, no. 7

Abstract

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ABSTRACT: In healthy cartilage, chondrocytes maintain an expression of collagens and proteoglycans and are sensitive to growth factors and cytokines that either enhance or reduce type II collagen synthesis. In osteoarthritis, pro-inflammatory cytokines, such as IL-6, induce overexpression of metalloproteinases (MMP) and decreasing synthesis of aggrecan. Use of chondroprotectors agents, such as Platelet-Rich Plasma (PRP) and triamcinolone (TA) are alternatives to reduce the progression of joint damage. In this study, we used chondrocytes extracted from metacarpophalangeal joints of healthy horses as the experimental model. Cells were treated in vitro with PRP or TA. No differences were observed between these treatments in comparison to the control group when the expressions of MMP9, MMP13, IL-6 and ACAN genes were evaluated (P<0.05). With these results, we can suggest that the treatments were not deleterious to equine cultured chondrocyte, once they did not stimulate MMPs and IL-6 synthesis or caused changes in ACAN.

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