Nature Communications (Jul 2021)
A synthetic nanobody targeting RBD protects hamsters from SARS-CoV-2 infection
- Tingting Li,
- Hongmin Cai,
- Hebang Yao,
- Bingjie Zhou,
- Ning Zhang,
- Martje Fentener van Vlissingen,
- Thijs Kuiken,
- Wenyu Han,
- Corine H. GeurtsvanKessel,
- Yuhuan Gong,
- Yapei Zhao,
- Quan Shen,
- Wenming Qin,
- Xiao-Xu Tian,
- Chao Peng,
- Yanling Lai,
- Yanxing Wang,
- Cedric A. J. Hutter,
- Shu-Ming Kuo,
- Juan Bao,
- Caixuan Liu,
- Yifan Wang,
- Audrey S. Richard,
- Hervé Raoul,
- Jiaming Lan,
- Markus A. Seeger,
- Yao Cong,
- Barry Rockx,
- Gary Wong,
- Yuhai Bi,
- Dimitri Lavillette,
- Dianfan Li
Affiliations
- Tingting Li
- State Key Laboratory of Molecular Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences (CAS)
- Hongmin Cai
- State Key Laboratory of Molecular Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences (CAS)
- Hebang Yao
- State Key Laboratory of Molecular Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences (CAS)
- Bingjie Zhou
- University of CAS
- Ning Zhang
- CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Center for Influenza Research and Early-warning (CASCIRE), CAS-TWAS Center of Excellence for Emerging Infectious Diseases (CEEID), CAS
- Martje Fentener van Vlissingen
- Erasmus Laboratory Animal Science Center, Erasmus University Medical Center
- Thijs Kuiken
- European Research Infrastructure on Highly Pathogenic Agents (ERINHA-AISBL)
- Wenyu Han
- State Key Laboratory of Molecular Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences (CAS)
- Corine H. GeurtsvanKessel
- European Research Infrastructure on Highly Pathogenic Agents (ERINHA-AISBL)
- Yuhuan Gong
- University of CAS
- Yapei Zhao
- University of CAS
- Quan Shen
- CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Center for Influenza Research and Early-warning (CASCIRE), CAS-TWAS Center of Excellence for Emerging Infectious Diseases (CEEID), CAS
- Wenming Qin
- National Facility for Protein Science in Shanghai, Shanghai Advanced Research Institute (Zhangjiang Laboratory), CAS
- Xiao-Xu Tian
- National Facility for Protein Science in Shanghai, Shanghai Advanced Research Institute (Zhangjiang Laboratory), CAS
- Chao Peng
- National Facility for Protein Science in Shanghai, Shanghai Advanced Research Institute (Zhangjiang Laboratory), CAS
- Yanling Lai
- State Key Laboratory of Molecular Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences (CAS)
- Yanxing Wang
- State Key Laboratory of Molecular Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences (CAS)
- Cedric A. J. Hutter
- Institute of Medical Microbiology, University of Zurich
- Shu-Ming Kuo
- CAS Key Laboratory of Molecular Virology & Immunology, Institut Pasteur of Shanghai CAS
- Juan Bao
- State Key Laboratory of Molecular Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences (CAS)
- Caixuan Liu
- State Key Laboratory of Molecular Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences (CAS)
- Yifan Wang
- State Key Laboratory of Molecular Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences (CAS)
- Audrey S. Richard
- European Research Infrastructure on Highly Pathogenic Agents (ERINHA-AISBL)
- Hervé Raoul
- European Research Infrastructure on Highly Pathogenic Agents (ERINHA-AISBL)
- Jiaming Lan
- CAS Key Laboratory of Molecular Virology & Immunology, Institut Pasteur of Shanghai CAS
- Markus A. Seeger
- Institute of Medical Microbiology, University of Zurich
- Yao Cong
- State Key Laboratory of Molecular Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences (CAS)
- Barry Rockx
- European Research Infrastructure on Highly Pathogenic Agents (ERINHA-AISBL)
- Gary Wong
- CAS Key Laboratory of Molecular Virology & Immunology, Institut Pasteur of Shanghai CAS
- Yuhai Bi
- University of CAS
- Dimitri Lavillette
- CAS Key Laboratory of Molecular Virology & Immunology, Institut Pasteur of Shanghai CAS
- Dianfan Li
- State Key Laboratory of Molecular Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences (CAS)
- DOI
- https://doi.org/10.1038/s41467-021-24905-z
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 13
Abstract
Here, the authors report the engineering, structural and biological characterization of synthetic nanobodies (sybodies) that display potent therapeutic activity against SARS-CoV-2 infection in animal models via targeting the virus receptor-binding domain.
